Naslov
Hypovirus CHV 1 in Croatia – basis for biological control of chestnut blight

Autori
Ćurković Perica, M. ; Krstin, Lj. ; Novak-Agbaba, S. ; Rigling, D. ; Krajačić, M

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of microbes in industry and environment 2007 / Kosalec, Ivan ; Pigac, Jasenka ; Vujaklija, Dušica - Zagreb : Hrvatsko mikrobiološko društvo, 2007

ISBN
978-953-96567-5-9

Skup
Power of microbes in industry and environment 2007

Mjesto i datum
Zadar, Hrvatska, 19.09.2007. - 22.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dsRNA; hypovirus; RFLP

Sažetak
The chestnut blight fungus, Cryphonectria parasitica (Murr.) Barr, is mainly responsible for the decline of the European chestnut (Castanea sativa Mill.) in Croatia. However, C. parasitica is a host for naturally occurring hypovirus which causes a reduction in fungal virulence and its reproductive capacities, enabling a biological control of the fungal pathogen. Since recovery of the chestnut forests is dependent on the natural occurrence of the hypovirulent C. parasitica strains in chestnut populations, we determined the incidence of hypovirulence in the central part of the country. Seventy eight isolates of the filamentous ascomycete C. parasitica were collected from 2004 to 2006, from four populations (Markuševac1, Markuševac 2, Samobor, Sljeme). Thirty four of them had white morphology, 19 intermediate, and 25 orange. Twenty six isolates (21 white, 2 intermediate and 3 orange) were tested for the presence of double stranded RNA (dsRNA) by cellulose chromatography and electrophoresis on agarose gels. Hypoviral dsRNA of approximately 12.7 kb in length was detected in all the white isolates tested. In 4 isolates M-dsRNA was also detected. The identity of the hypoviral isolates was determined by reverse transcription, polymerase chain reaction of two hypoviral open reading frames and restriction fragment length polymorphism analysis. Restriction patterns of the amplified products showed little variation among the examined hypoviruses from Croatia. Six patterns were completely identical with that of a hypoviral isolate from a fungus collected in Croatia 22 years ago. Phylogenetic analysis based on the restriction patterns grouped all hypoviruses from Croatia to the subtype I of CHV-1.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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