Naslov
Vegetativno razmnožavanje vrste Epimedium alpinum L. u uvjetima in vitro
(In vitro propagation of Epimedium alpinum L.)

Autori
Mihaljević, Snježana ; Vršek, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

ISBN
978-953-99774-1-0

Skup
2. hrvatski botanički kongres s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 20.09.2007. - 22.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
kultura tkiva; organogeneza; regeneracija
(organogenesis; regeneration; tissue culture)

Sažetak
The genus Epimedium (bishop’ s hat, barrenwort) in the family Berberidaceae comprises about 60 species of rhizomatous perennials. A number of species, hybrids, and cultivars are grown as ornamentals in woodland gardens and naturalized areas. In Croatia, Epimedium alpinum L. has the status of a vulnerable species endangered by uncontrolled collecting. The plant can be propagated by division of its long rhizomes or by seeds. The difficulties associated with gathering the seeds and low seed viability make bishop’ s hat a suitable species for propagation by tissue culture. In an attempt to develop a system for micropropagation of E. alpinum, the effects of various concentrations of thidiazuron (TDZ) and N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU), on the induction of organogenic callus was evaluated. Organogenesis from immature seeds occurred most efficiently when they were transiently exposed (48 hours) to 20 &micro ; M CPPU or 80 &micro ; M TDZ followed by culture on hormone-free woody-plant medium (WPM). Organogenic callus consisting of white, compact clumps of tissue proliferated slowly on hormone-free WPM. To promote adventitious shoot induction, the effects of different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzyladenine (BA) were investigated. WPM containing 1.1 &micro ; M 2, 4-D and 4.4 &micro ; M BA was optimal for callus proliferation while shoot induction and elongation was enhanced on media with 1.1 &micro ; M 2, 4-D and 2.2 or 22 &micro ; M BA. Shoots were successfully rooted on hormone-free WPM and well-developed plantlets were transferred to soil.

Izvorni jezik
Hrvatski

Znanstvena područja
Biologija

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