Naslov
Laccase catalysed oxidation of L-DOPA in a microreactor

Autori
Tišma, Marina ; Žnidaržič-Plazl, Polona ; Plazl, Igor ; Zelić, Bruno ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th International symposium on biocatalysis and biotransformations / - , 2007

Skup
Biotrans 2007

Mjesto i datum
Oviedo, Španjolska, 08.07.2007. - 13.07.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
L-DOPA; laccase; microreactor

Sažetak
Laccases (EC 1.10.3.2 p-diphenol: dioxygen oxidoreductases) are multi-copper proteins that use molecular oxygen to oxidize various aromatic and non-aromatic compounds by radical-catalyzed reaction[1]. Their applications are numerous: delignification or decolouration of paper pulp, detoxification of environmental pollutants, textile dye-bleaching or diagnostic assays[2]. The fields of application of enzymatic microreactors grow every day. Their current use is primarily in biochemical analysis as components of integrated systems (lab-on-a-chip) or in micro total analysis systems - &micro ; ; TAS. Secondly, microreactors are used for screening of substrates, enzymes and to examine their kinetic characteristics. However, their huge potential is not yet exploited in biotechnology as well as in combinatorial chemistry and enzyme-targeted drug search[3]. In this work, the use of microreactor for oxidation of phenolic compounds was investigated. L-DOPA was selected as a model substrate for laccase-catalysed oxidation, which was carried out in a microreactor (220 &micro ; ; m X 50 &micro ; ; m X 0.332 cm) at four different residence times. Process model, comprised of reaction diffusion dynamics combined with fluid flow was developed. The simulation of the process for different substrate and enzyme concentrations, as well as for different flow rates was performed. A very good correlation of experimental data with the proposed model was achieved. Literature: [1] Claus H.: Laccases: structure, reactions, distribution ; Micron 35 (2004), 93-96 [2] Pilz R., Hammer E., Schauer F., Kragl U.: Laccase – catalysed of coupling products of phenolic substrates in different reactors: Appl Microbiol 60 (2003), 708-712 [3] Urban, P. L., Goodall D. M., Bruce N. C.: Enzymatic microreactors in chemical analysis and kinetic studies, Biotechnol Adv 24 (2006), 42-57

Izvorni jezik
Engleski

Znanstvena područja
Drvna tehnologija

POVEZANOST RADA