Naslov
Streptomyces rimosus proteases: properties and use
(Streptomyces rimosus proteases: propereties and use)

Autori
Vitale, Ljubinka ; Renko, Metka ; Lenarčić, Boris ; Pokorny, Mišo ; Turk, Vito ; Šimaga, Šumski ; Vukelić, Bojana ; Grdiša, Mirica ; Abramić, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Sixth Regional Meeting of Biochemists and Molecular Biologists

Mjesto i datum
Trst, Italija, 06.10.1983. - 08.10.1983

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Streptomyces species; proteolytic enzymes

Sažetak
Constantly increasing possibilities of proteolytic enzymes application, create a need for the new types of enzymes. A good source to search for them are Streptomyces species known to synthesize a great veriety of proteases. From Streptomyces rimosus culture filtrates waste waters of antibiotic production, a crude enzyme mixture and 4 electrophoretically homogenous enzymes were prepared. Molecular weight, isoelectric point, stability, pH and temperature optimum, preferential substrates and susceptibility to synthetic and natural inhibitors, were determined for the isolated enzymes. Their properties suggest classification as leucine aminopeptidase, chymotrypsin-like, trypsin-like and alkine metallo proteinase. Crude enzyme mixture was used as an active component for the bating agent Encipon, whose performance was compared with that of pancreatic and Bacillus enzymes preparations. Analysis of degradation products and leather quality have shown that S. rimosus enzymes are suitable for hides and skins treatment.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA