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Pregled bibliografske jedinice broj: 302193

Mathematical modelling of the NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in the continuously operated enzyme membrane reactor

Findrik, Zvjezdana; Vrsalović Presečki, Ana; Vasić-Rački, Đurđa
Mathematical modelling of the NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in the continuously operated enzyme membrane reactor // Journal of bioscience and bioengineering, 104 (2007), 4; 275-280 doi:10.1263/jbb.104.275 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 302193 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Mathematical modelling of the NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in the continuously operated enzyme membrane reactor

Autori
Findrik, Zvjezdana ; Vrsalović Presečki, Ana ; Vasić-Rački, Đurđa

Izvornik
Journal of bioscience and bioengineering (1389-1723) 104 (2007), 4; 275-280

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NADH oxidase ; coenzyme regeneration ; enzyme kinetics ; enzyme membrane reactor ; inhibition

Sažetak
NADH oxidase from Lactobacillus brevis was kinetically characterized in two different buffers: Tris HCl and glycine-sodium pyrophosphate pH 9.0. Reaction kinetics was described by Michaelis-Menten model with product, NAD+, inhibition. It was found that the type of this inhibition is uncompetitive. Experiments in the continuously operated enzyme membrane reactor revealed high enzyme deactivation at two different residence times: 12 and 60 min. Higher deactivation was noticed at the lower residence time in glycine-sodium pyrophosphate buffer. Enzyme deactivation was assumed to be of the first order. The developed mathematical model for the continuously operated enzyme membrane reactor described these experiments quite well. Mathematical model simulations revealed that high enzyme concentration (up to 30 g cm-3) is necessary to obtain and keep the stationary NADH conversion near 100 % through the longer period of time.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija



POVEZANOST RADA

Projekti:
MZOS-125-1252086-2793 - Biokatalizatori i biotransformacije (Vasić-Rački, Đurđa, MZOS ) ( CroRIS)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Avatar Url Đurđa Vasić-Rački (autor)

Avatar Url Ana Vrsalović Presečki (autor)

Avatar Url Zvjezdana Findrik Blažević (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com dx.doi.org

Citiraj ovu publikaciju:

Findrik, Zvjezdana; Vrsalović Presečki, Ana; Vasić-Rački, Đurđa
Mathematical modelling of the NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in the continuously operated enzyme membrane reactor // Journal of bioscience and bioengineering, 104 (2007), 4; 275-280 doi:10.1263/jbb.104.275 (međunarodna recenzija, članak, znanstveni)
Findrik, Z., Vrsalović Presečki, A. & Vasić-Rački, Đ. (2007) Mathematical modelling of the NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in the continuously operated enzyme membrane reactor. Journal of bioscience and bioengineering, 104 (4), 275-280 doi:10.1263/jbb.104.275.
@article{article, author = {Findrik, Zvjezdana and Vrsalovi\'{c} Prese\v{c}ki, Ana and Vasi\'{c}-Ra\v{c}ki, \DJur\dja}, year = {2007}, pages = {275-280}, DOI = {10.1263/jbb.104.275}, keywords = {NADH oxidase, coenzyme regeneration, enzyme kinetics, enzyme membrane reactor, inhibition}, journal = {Journal of bioscience and bioengineering}, doi = {10.1263/jbb.104.275}, volume = {104}, number = {4}, issn = {1389-1723}, title = {Mathematical modelling of the NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in the continuously operated enzyme membrane reactor}, keyword = {NADH oxidase, coenzyme regeneration, enzyme kinetics, enzyme membrane reactor, inhibition} }
@article{article, author = {Findrik, Zvjezdana and Vrsalovi\'{c} Prese\v{c}ki, Ana and Vasi\'{c}-Ra\v{c}ki, \DJur\dja}, year = {2007}, pages = {275-280}, DOI = {10.1263/jbb.104.275}, keywords = {NADH oxidase, coenzyme regeneration, enzyme kinetics, enzyme membrane reactor, inhibition}, journal = {Journal of bioscience and bioengineering}, doi = {10.1263/jbb.104.275}, volume = {104}, number = {4}, issn = {1389-1723}, title = {Mathematical modelling of the NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in the continuously operated enzyme membrane reactor}, keyword = {NADH oxidase, coenzyme regeneration, enzyme kinetics, enzyme membrane reactor, inhibition} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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  • CA Search (Chemical Abstracts)
  • Excerpta Medica

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