Naslov
Effluents from oil production platforms in the North Sea inhibit the function of the ABC transporters MDR1 and MRP1 in vitro

Autori
Pivčević, Branka ; Smital, Tvrtko ; Tollefsen, Knut-Erik

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Pollutant responses in marine organisms ; 14th International symposium / PRIMO Advisory Board - Florianopolis : Universidade Federal de Santa Catalina, Brazil, 2007

Skup
Pollutant responses in marine organisms ; 14th International symposium

Mjesto i datum
Florianópolis, Brazil, 06.07.2007. - 09.07.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ABC inhibitors; MDR1; MRP1; calcein-AM bioassay; HL60; oil; produced water; dispersed oil; water soluble fraction; solid phase extraction

Sažetak
Inhibition of multixenobiotic resistance (MXR) defence by various environmental contaminants may in aquatic organisms cause significant increase in intracellular accumulation and toxic effects of other xenobiotics normally effluxed by ABC transport proteins. In this study the inhibition potency of production water samples from North Sea offshore oil platforms on the efflux activity of the ABC transporters Pgp1 (ABCB1) and MRP1 (ABCC1) was examined using an in vitro calcein-AM bioassay with HL60 cells stably transfected with either human ABCB1 or ABCC1 genes. Transfected cells are characterized by an efficient efflux and low intracellular accumulation of calcein-AM, which can be modulated by numerous different inhibitors. The inhibition potency of the samples was expressed as inhibiting equivalents (EQ) of the model inhibitors reversin 205 (REV) for Pgp1 and MK571 for the MRP1 transporters. Production water samples were collected from ten oil and gas production platforms with different chemical composition, extracted by large volume solid phase extraction, and prepared as a particulate/oil fraction and a water soluble fraction. The majority of samples had measurable concentrations of Pgp1 inhibitors (range: 37 – 380 &micro ; ; g/L REV EQs), and the Pgp1 inhibiting activity was predominantly found in the water fraction of produced water. MRP1 inhibitors were determined in all samples with highest concentration in extracts of particulate/oil fraction of produced water (range: 88 – 1400 &micro ; ; g/L MK571 EQs). These results indicate that there are more inhibitors of MDR1 than of Pgp1 transporter in produced water, and that the active chemicals exhibit different physico-chemical properties that may affect the partition behaviour in the environment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA