Naslov
Trypanosomes of the subgenus Megatrypanum: parity between spliced leader and ribosomal sequences supported lineages divergence according to host restriction with artiodactyl species

Autori
Adriana C. Rodrigues , Jael S. Batista, Franjo Martinković, Marta Campaner, Marta M. G. Teixeira

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXII Annual Meeting of the Brazilian Society of Protozoology / XXXIII Annual Meeting on Basic Research in Chagas Disease / - , 2006, 96-96

Skup
XXII Annual Meeting of the Brazilian Society of Protozoology / XXXIII Annual Meeting on Basic Research in Chagas Disease

Mjesto i datum
São Paulo, Brazil, 06.11.2006. - 08.11.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Trypanosoma theileri

Sažetak
Trypanosoma theileri and related species of the subgenus T. (Megatrypanum) from cattle and water buffalo isolated from distant Brazilian geographic regions (North, Northeast, Central and Southeast) were used to evaluate genetic relatedness based on polymorphisms of the spliced leader (SL) gene. SL repeats showing high similarity of length and sequences were shared for all T. theileri trypanosomes, a clade including only trypanosomes from artiodactyls. In contrast, length and sequence of SL were very variable among Megatrypanum species of other mammalian order than Artiodactyla. Dendrogram constructed based on SL transcript (exon and intron) sequences were analyzed aiming to evaluate the degree of genetic relatedness among these organisms. Trypanosomes of European cattle, deer and sheep used for comparison were not clustered with Brazilian isolates. Phylogenies using SSU rRNA gene presented genetic divergences that suggested an ancestral origin for bovids and cervids trypanosomes. Moreover, results supported parity between SL and ribosomal (SSU and ITS1) sequences, with total congruency of lineages. Taken together, T. theileri trypanosomes from Brazil and Europe were segregated into six lineages, with cattle isolates distributed into three lineages (B, C, D) separated from lineages of isolates from buffalo (A), deer (E) and sheep (F). Thus, segregation pattern of T. theileri trypanosomes supported the hypothesis of lineages divergence according to their artiodactyl hosts. Moreover, parity between lineages defned by SL and ribosomal gene markers suggested clonal structure of parasite populations, with lineages associated to geographical origin of isolates. In conclusion, isolates from different artiodactyl hosts, as well as isolates from cattle living separated by a large geographical distance, were not clustered together.

Izvorni jezik
Engleski

POVEZANOST RADA