Naslov
Biotransformation of D-methionine into L-methionine in the cascade of four enzymes

Autori
Findrik, Zvjezdana ; Vasić-Rački, Đurđa

Izvornik
Biotechnology and bioengineering (0006-3592) 98 (2007), 5; 956-967

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
enzyme kinetics ; L-phenylalanine dehydrogenase ; D-amino acid oxidase ; formate dehydrogenase

Sažetak
D-methionine was converted to L-methionine in a reaction system where four enzymes were used. D- amino acid oxidase from Arthrobacter protophormiae was used for the complete conversion of D- methionine to 2-oxo-4-methylthiobutyric acid. Catalase was added to prevent 2-oxo-4- methylthiobutyric acid decarboxylation. In the second reaction step L- phenylalanine dehydrogenase from Rhodococcus sp. was used to convert 2-oxo-4- methylthiobutyric acid to L- methionine, and formate dehydrogenase from Candida boidinii was added for NADH regeneration. Enzyme kinetics of all enzymes was analyzed in detail. Mathematical models for separate reactions steps, as well as for the complete system were developed and validated in the batch reactor experiments. Complete conversion of D-methionine to L- methionine was achieved. Considering that both enzymes act on different substrates, such a system could be easily employed for the synthesis of other amino acids from L-isomer, as well as from the racemate of a certain amino acid (DL-amino acid).

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA