Naslov
Sialidase and chondroitinase enhance spinal axon outgrowth into peripheral nerve grafts in a rat model of brachial plexus injury

Autori
Lorenzini, Ileana ; Vajn, Katarina ; Schramm, Lawrence P. ; Schnaar, Ronald L. ; Yang, Lynda JS.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Skup
Meeting of Society for Neuroscience

Mjesto i datum
Washington D.C., Sjedinjene Američke Države, 12.11.2005. - 16.11.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sialidase; chondroitinase; brachial plexus injury

Sažetak
Axon regeneration inhibitors, including chondroitin sulfate proteoglycans, Nogo, OMgp, and myelin-associated glycoprotein (MAG) on myelin and/or astroglia, limit recovery from spinal cord injuries, including brachial plexus (nerve root) avulsion. Among axonal ligands for MAG are sialoglycoconjugates, particularly gangliosides GD1a and GT1b. Sialidase treatment reverses MAG's inhibition of axon regeneration in vitro. We now report the in vivo contribution of sialoglycoconjugates in limiting axon regeneration using a rat model of brachial plexus avulsion injury. The C8 ventral root underwent intradural disruption with subsequent insertion of a peripheral nerve graft at the site. This resulted in modest spinal axon outgrowth into the graft. Saline alone (control), sialidase in saline (0.4 or 0.1 U/ml) or chondroitinase ABC in saline (0.5 U/ml) was delivered to the site of graft insertion for two weeks via an osmotic pump. Subsequently, spinal axons extending well into the peripheral nerve graft were retrogradely labeled with a fluorescent dye. Tissues were fixed by transcardiac perfusion with paraformaldehyde, the spinal cord was dissected, and axon outgrowth into the graft was quantified by measuring the number of fluorescently labeled spinal neurons. Marked and significant (p < 0.01) enhancement of axon outgrowth into the peripheral nerve graft was observed in animals treated with 0.4 U/ml sialidase (2.6-fold) or chondroitinase (2.5-fold), whereas 0.1 U/ml sialidase was without effect. These results indicate that sialidase enhances axon regeneration and that sialidase treatment may aid functional recovery after nervous system injury or disease.

Izvorni jezik
Engleski

POVEZANOST RADA