Naslov
Does transduced p27 induced apoptosis in human tumor cell lines?

Autori
Grdiša, Mira ; Mikecin, Ana-Matea ; Poznić, Miroslav

Izvornik
Annals of the New York Academy of Sciences (0077-8923) 1090 (2006); 120-129

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
p27 ; apoptosis ; cell lines

Sažetak
P27 is a cyclin dependent kinase inhibitor, involved in the negative regulation of G1 progression in response to a number of anti-proliferative signals. In this study the transduction of full-length Tat-p27, pt-mutated Tat-p27 and N'- Tat-p27 (truncated p27 on the C-terminal end) fusion proteins into human tumor cell lines was examined and whether these transduced proteins induced apoptosis in the cells. Protein transduction can be described as the direct uptake by the cell of exogenous proteins/peptides, as a result of a specific property of the protein/peptide component. The basic domain of human immunodeficiency virus type 1 (HIV-1) transactivator of transcription (Tat) protein possess the ability to traverse biological membranes efficiently in a process termed « ; protein transduction» ; . Although the mechanism is unknown, transduction occurs in receptor-transporter-independent manner that appears to target the lipid bilayer directly. Thus, HIV-1 Tat proteins have tramendous potential to deliver large-sized compounds into the cells. Transduction of TAT-fusion proteins affected the proliferation of human tumor cell lines, depending on the type of protein and cell line. By Western blot analysis was shown that some cell cycle regulatory proteins were affected, as well as some proteins responsable for induction of apoptosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
Sv. 1090 : Signal Transduction Pathways. Part A : Apoptotic and Extracellular Signaling

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