ࡱ> 5@bjbj22, XXZZZ8  D F F F F F F $ RLj -j  D D     tx"Zud  , 0 11 1 Dj j Z ZBIOTRANSFORMACIJA D-METIONINA U L-METIONIN U VI`EENZIMSKOM SUSTAVU BIOTRANSFORMATION OF D-METHIONINE TO L-METHIONINE IN MULTIENZYME SYSTEM Zvjezdana Findrik, ura Vasi-Ra ki Fakultet kemijskog in~enjerstva i tehnologije, Savska c. 16, 10 000 Zagreb, HRVATSKA Enantiomerno iste aminokiseline imaju zna ajnu ulogu kao aktivni sastojci ili klju ni dijelovi proizvoda agroindustrije, farmaceutske, kozmeti ke industrije, te industrije ljudske i sto ne hrane [1]. Takoer se koriste u pripremi pomonih komponenti, induktora, liganada i katalizatora za asimetri ne sinteze. Do sada je razvijen niz procesa za sintezu enantiomerno istih aminokiselina. U ovom su radu provedene reakcija oksidacije D-metionina i redukcije produkta 2-okso-4-metiltiomasla ne kiseline u L-metionin. U sustavu su bila prisutna etiri enzima: oksidaza D-aminokiselina iz Arthrobacter protophormiae za oksidaciju D-metionina, L-fenilalanin dehidrogenaza iz Rhodococcus sp. za redukciju 2-okso-4-metiltiomasla ne kiseline, formijat dehidrogenaza "   . : L T V ` b      * . J L .Pf&JbdfjnprſŴūŜŜťń hQ7CJU h8$CJhXh ]6CJhXh ]CJ h ]CJhb1h ]CJh ]h3CJaJ h^ CJ h3CJhJh3hJh^ CJ$aJ$ h3CJ hCJ h8$CJhCJaJh8$CJaJ0  b    r$$a$gd ]$d^`a$gd3$a$gdQ7 $xa$gdJ$a$ $dxa$gdJiz Candida boidinii za regeneraciju NADH i katalaza iz govee jetre za uklanjanje vodikovog peroksida nastalog reakcijom oksidacije D-metionina. Reakcijska shema je prikazana na slici.  EMBED ISISServer  U svim spomenutim reakcijama u sustavu je odreivana kinetika metodom po etnih brzina, tj. mjerenjem utjecaja pojedinih reakcijskih komponenti na po etnu reakcijsku brzinu. Na temelju eksperimentalnih podataka su procijenjene vrijednosti parametara u kineti kom izrazu, te je razvijen matemati ki model za aar~ni reaktor. Provedeni su aar~ni eksperimenti kojima su verificirani matemati ki modeli za pojedine reakcijske stupnjeve, te za ukupnu reakciju. Dobiveno je dobro slaganje matemati kog modela i eksperimentalnih podataka, te je pokazano da navedeni enzimi mogu uspjeano katalizirati navedene reakcije bez da interferiraju jedan drugom. Postignuta je 100 %-tna konverzija D-metionina u L-metionin. [1] Drauz K., Eils S., Schwarm M., Chimica oggi, 2002, January/February. rt(*$,jĺİӪhJ hcHCJh9h ]5CJh9h ]6CJhb|h ]CJ hCJ h ]CJ h3CJh ]h3CJjtH hlUVhljhlU*1h:p^ . 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