Naslov
Estimation of smoking effect on genome of human lymphocytes exposed to gamma radiation using micronucleus assay

Autori
Garaj-Vrhovac, Vera ; Franekić Čolić, Jasna ; Viculin, Tomislav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the International Conference of Oxidative Stress : Havana-Redox 2007 ; u: Revista Cubana de Farmacia 41 Supplement (2007) (S1) / - , 2007, 36-36

Skup
International Conference of Oxidative Stress : Havana-Redox 2007

Mjesto i datum
Havana, Kuba, 25.01.2007. - 27.01.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biomarkers; DNA damage; smoking; micronucleus assay

Sažetak
Using micronucleus assay we investigated genotoxicity of smoking on genome of human lymphocytes in spontaneous in vivo conditions and gamma radiation induced in vitro conditions. Peripheral blood sample was obtained from a healthy non-smoking and smoking donors (age 20-25 years). One blood sample from each donors were exposed to gamma rays from 60Co source (Alcyon CGR-MeV). Total exposure to radiation lasted 1.24 minutes, and the absorbed dose was 2 Gy. Other blood samples were not irradiated, but they were handled in the same manner. From each blood sample lymphocyte cultures for the cytokinesis blocked micronucleus assay was established. Micronucleus assay established that the degree of damage of genome in smokers lymphocytes was higher compared to nonsmokers and the difference reached statistical significance. However, differences in total number of micronuclei of both donors, male and female donors were not found. The results of the present study indicate inter-individual differences in DNA damage between donors, indicating differences in genome radiosensitivity.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA