Naslov
Preparation and characterization of simple amperometric biosensor for glucose with alkali nickel hexacyanoferrate(II) electrocrystallized on nickel electrode and glucose oxidase immobilized in bovine serum albumin cross-linked with glutaraldehyde

Autori
Milardović, Stjepan ; Mikšaj, Miroslav ; Kruhak, Ivan ; Filipović Kovačević, Željka ; Grabarić, S. Božidar

Izvornik
Food technology and biotechnology (1330-9862) 34 (1996), 4; 153-159

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
biosensor for glucose ; alkali nickel hexacyanoferrate ; nickel electrode ; glucose oxidase

Sažetak
Preparation and characterization of a simple amperometric enzyme-based glucose sensor is reported. The enzyme glucose oxidase is immobilized in bovine serum albumin by cross-linking with glutaraldehyde on the layer of alkali nickel hexacyanoferrate(II) electrocrystallized on the surface of nickel electrode. The selective biocatalytic effect of the enzyme glucose oxidase on the oxidation of glucose and catalytic effect of nickel electrode modified with alkali nickel hexacyanoferrate(II) on the reduction of hydrogen peroxide, produced by the enzymatic reaction, enables the amperometric glucose biosensor to operate using working potentials between -300 and -100 mV vs. SCE, where the oxidation of most important interferences present in human blood (ascorbic or uric acidi is not possible. Other characteristics of the reported biosensor are: practically independent current response of pH in the range between 6.7 and 7.7, response time for 0.3 mM glucose addition in sodium phosphate buffer solution (0.01 M phosphate ion, pH = 7.4) and 0.1 M of sodium perchlorate shorter than 20 s, relatively long time stability (during three months of operation the sensitivity decreased only 30% of the initial sensitivity) and linear range of glucose concentration up to 1.2 mM.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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