Naslov
The Distribution of the Populations of Sessile Oak (Quercus petraea (Matt.) Liebl) in Bosnia and Herzegovina by Chloroplast DNA (cpDNA) and its Application in Forestry

Autori
Ballian, Dalibor ; Ivanković, Mladen ; Slade, Danko

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Forstliche Genressourcen als Produktionsfaktor / - Hessen, 2006, 121-131

Skup
26.Tagung der Arbeitsgemeinschaft Forstgenetik und Forstpflanzenzuchtung

Mjesto i datum
Fuldatal, Njemačka, 20.10.2005. - 22.10.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Quercus petraea; sessile oak; variability; cpDNA

Sažetak
The genetic variability of sessile oak (Quercus petraea) was investigated by cpDNA analysis, with application of AFLP technique. We determined the haplotypes of 16 populations, their correlations and relations to other populations from Europe. The analysis showed that in the researched populations of sessile oak in Bosnia and Herzegovina there exists seven different haplotypes (2, 4, 5, 6, 7, 17, 31). In the haplotype 5 there were determined three previous sub haplotypes (a, b and c), and in 31 two (a and b). The cpDNA polymorphism was not under the selection pressure, and the cpDNA structure was therefore preserved from the glacial period until today, as opposed to the genetic markers mainly placed in the nucleuses, which have entirely lost their “ initial” geographic structure since the glaciation period. The results obtained by this analysis point at the presence of large variability in Bosnia and Herzegovina, which is also typical for other species of the Dinaric Alps. Identification of the populations in the broader area by analysis of haplotypes could in the future, since our work achieved good results. This method perceives the analysis of additional populations, in order to obtain as precise overview of the distribution of separate haploid types in Bosnia and Herzegovina as possible. In that way we hope that it will be possible to determine the origin of planting material, that is, to eliminate the populations from which it most likely does not originate. That would considerably decrease the possibility of speculations as well as the transfer of the planting material between different areas. The results obtained by the cpDNA analysis can also help in forming of seed zones, but due to high selection pressure against sessile oak by humans through an unplanned cutting, and by introduction of plant material of an unknown origin, it is necessary to find a sufficient number of autochthonous units, that is, populations of the investigated species, as well as new appropriate markers for their characterization. Based on the results of this research we could, in a scientifically recognized way, determine the origin of the initial planting material from a broader geographic area, which would serve the reproductive purpose.

Izvorni jezik
Engleski

Znanstvena područja
Šumarstvo

POVEZANOST RADA