Naslov
Ultrastructural features in differential diagnosis of tumours

Autori
Špoljar, Mira ; Čužić, Snježana ; Hlupić, Ljiljana ; Grandić, Nevenka ; Majsec, Dubravka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of the 2nd Croatian congress on pathology and forensic medicine / Anđelinović, Šimun - Split : Hrvatsko društvo patologije i sudske medicine HLZ, 2000

Skup
2nd Croatian congress on pathology and forensic medicine with international participation

Mjesto i datum
Split, Hrvatska, 21.09.2000. - 23.09.2000

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Tumours; electron microscopy; cytoplasmic organelles; specific inclusions

Sažetak
Electron microscopy(EM)has quite often an essential role in the diagnosis of certain tumours and some other diseases, for example Langerhans cell histiocytosis.Ultrastructural analysis is especially important in the differential diagnosis of poorly differentiated mesenchymal and occasionally epithelial tumours, when immunohistochemistry is negative or dubious. This study is based on the investigation of over 250 various tumours that have been sent to our laboratory during the period of the last 15 years.All the material was primarily fixed in 10% buffered formaldehyde, postfixed in 1% osmium tetroxyde for 2 hours and then left overnight in 3% uranyl acetate(UA)for better contrast.For rinsing of tissue phosphate buffer with pH 7.3 was used in all cases.Dehydration was performed in series of acetones and then the tissue was embedded in Durcupan.Ultrathis sections were stained with 4% UA and lead citrate, or by hot UA at 90 oC(When we wanted to study microfilaments), and then analyzed and photographed by electron microscope OPTON EM 9A. We wanted to show the cytoplasmic organelles and various components of cytosceleton and extracellular matrix which are specific for certain tumours or represent the ultrastructural signs of tumour differentiation.They include some typical organelles and modifications of tumour cell surface, various types of microfilaments and some characteristic cytoplasmic or extracellular inclusion bodies that were in most cases the crucial finding for the final diagnosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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