Pregled bibliografske jedinice broj: 284413
THE ROLE OF PKC α and δ IN INSULIN SIGNALING IN RAT HEPATOCYTE
THE ROLE OF PKC α and δ IN INSULIN SIGNALING IN RAT HEPATOCYTE // FEBS Special Meeting ; Cellular Signaling-Dubrovnik 2006 / Đikić, Ivan ; Husnjak, Koraljka (ur.).
Zagreb, 2006. str. 111-112 (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
THE ROLE OF PKC α and δ IN INSULIN SIGNALING IN RAT HEPATOCYTE
Autori
Roša, Jagoda ; Gabrić, Janko ; Galić, Irena ; Roša, Josip
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
FEBS Special Meeting ; Cellular Signaling-Dubrovnik 2006
/ Đikić, Ivan ; Husnjak, Koraljka - Zagreb, 2006, 111-112
Skup
FEBS Special Meeting ; Cellular Signaling-Dubrovnik 2006
Mjesto i datum
Dubrovnik, Hrvatska, 26.05.2006. - 01.06.2006
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
hepatocytes; insulin signaling; protein kinasa C (PKC) α and δ
Sažetak
Aims. The binding of insulin to its receptor induces a cascade of events leading to activation of downstream elements. One of important component of this cascade is the protein kinase C (PKC) family of serine-treonine kinases. The aim of this investigation was to determine the possible role of PKC α and δ in insulin signal pathway and in insulin resistance. Methods. Hepatocytes were isolated by a modified collagenase perfusion technique of Berry and Friend and cultured for 24 h in the serum free M199 medium without or with (0.08 umol/l) insulin. For study AIB transport cells were incubated for one hour in Hanks-hepes medium containing 2 g/l albumin and alfa-amino isobutyric acid (AIB) in the absence and presence of insulin. PKC α and δ stimulator (TPA), PKC α inhibitor GO6976 and PKC δ inhibitor rottlerin were added to the medium before insulin treatment. Results. In hepatocytes obtained from rats on standard diet insulin produced almost 100% increase of AIB transport . Activation of PKC α did not change basal AIB transport but strongly reduced insulin effect (> 50%). On the other side inhibition of PKC-α did not change basal as well as insulin stimulated AIB transport. Inhibition PKC-δ with rottlerin totaly abolished insulin effect and the activation of PKC-δ mimic insulin effect. Conclusion. Since PKC-δ is constitutively associated with insulin receptor, the effect of insulin on AIB transport in hepatocytes occurs probably through activation of PKC-δ . Role of PKC-α in insulin signaling is not clear but it activation can be responsible for developing of insulin resistance.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
