Pregled bibliografske jedinice broj: 283966
Use of proteolytic enzymes immibilized on monolithic CIM disks for rapid analytical and preparattive cleavage of proteins
Use of proteolytic enzymes immibilized on monolithic CIM disks for rapid analytical and preparattive cleavage of proteins // 24International Symphosium on the Separation of Proteins, Peptides and Polynucleotides
Aachen, Njemačka, 2004. (poster, nije recenziran, sažetak, znanstveni)
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Naslov
Use of proteolytic enzymes immibilized on monolithic CIM disks for rapid analytical and preparattive cleavage of proteins
Autori
Y-P.Lim, M.Ručević, H.Callanan, M.K.Brown, D.C.Hixon, D.Josić
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
24International Symphosium on the Separation of Proteins, Peptides and Polynucleotides
Mjesto i datum
Aachen, Njemačka, 19.10.2004. - 22.10.2004
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
enzymes; CIM disks
Sažetak
So called Convective Interaction Media (CIM) monolithic disks offer a simple and rapid method for proteolythic cleavage of investigated proteins. We used several proteolytic enzymes such as trypsin, elastase and granzyme immobilized on CIM disks of various sizes for digestion of standard proteins and different samples with unknown proteins. Proteolytic fragments can be subsequently separated by use of SDS-PAGE or RP-HPLC. For RP-HPLC particle-based or disk-shaped chromatographic columns can be used. Unknown proteins can be identified by Western blot, SELDI-TOF technique or by use of mass spectrometry. The immobilized enzymes on the Connective Interaction Media are stable and still active after repeated runs. A partial or complete digestion can be achieved by varying the flow rate.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
