Naslov
Specific features of Picea abies L. (Karst.) vegetative buds during winter-spring period

Autori
Cesar, Vera ; Cesar, Dobroslav

Izvornik
Acta botanica Croatica (0365-0588) 47 (1988); 248-248

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
Picea abies; Vegetative buds; In-vitro culture; Anatomy

Sažetak
Recent studies of P. abies vegetative buds have shown that one part of the bud, the crown, has an influence on the transport of some substances and consequently has an effect on the growth of vegetative buds in in-vitro culture. This paper presents part of investigations into the morfological and anatomical changes of the P. abise vegetative bud during winter-spring period. Dormant vegatative buds of P. abies were taken weekly from four-year-old trees at Hilleshog AB nursery, South Sweden, for 11 weeks from the middle of February to the end of April in 1985. Buds for anatomical observations and measurement were isolated from scales and fixed in glutaraldehyde, dehydrated and fitted in thermoplastic resin. Longitudinal sections, 3 μm thick, were made and stained with toluidine blue. Data obtained by measurement were statistically processed. Vegetative buds of P. abies have three parts: (1) embryonal cells which produce spring shoot, (2) the crown made up of small, densely congested thick wall cells and (3) the cavity, bordered with long cells and filled with loose cells. Transport tissue from the branch reaches the lower margin of the cavity. The area and height of these structures of two different clones were measured throughout the 11 weeks. Significance tested of the ratio difference in the areas of structures 1/2, 1/3, 3/2 showed that there was a significant difference in increase of ratios 1/2 and 1/3 in the middle of April (spring). This difference did not exist for the ratio 3/2, what indicates the sudden growth of structure 1 area. Similar results were obtained by testing the height of the same structures. Results for both investigated clone coincided.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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