Naslov
Changes of heterotic pattern and allele frequencies in the M3S maize population after two cycles of selfed progeny recurrent selection revealed by SSR markers

Autori
Šarčević, Hrvoje ; Pejić, Ivan ; Barić, Marijana ; Kozumplik, Vinko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts XIII EUCARPIA Biometrics in Plant Breeding Section Meeting / Gunjača, Jerko ; van Eeuwijk, Fred A. ; Šatović ; Zlatko - Zagreb : Agronomski fakultet Sveučilišta u Zagrebu, 2006

Skup
XIII EUCARPIA Biometrics in Plant Breeding Section Meeting

Mjesto i datum
Zagreb, Hrvatska, 30.08.2006. - 01.09.2006

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
maize; exotic germplasm; recurrent selection; allele frequency; heterotic pattern

Sažetak
Maksimir 3 Synthetic (M3S) maize population was developed at the Faculty of Agriculture University of Zagreb by intercrossing inbred lines, which origin traces back to several open-pollinated varieties and local populations from different regions of the former Yugoslavia. The population was subjected to two cycles of selfed progeny recurrent selection, which resulted in a significant increase in grain yield of 12% per cycle with no significant changes in lodging resistance. The objectives of this study were: i) to determine genetic relationship among the parental inbred lines of the M3S population (M3S progenitors), the M3S population, and elite inbred lines representing the BSSS and Lancaster heterotic groups and ii) to examine the effect of two cycles of recurrent selection on allele frequency changes in the population. Nine M3S progenitors, three BSSS lines, and three Lancaster lines were genotyped at 24 SSR loci, out of which nine randomly chosen loci were used for genotyping 96 individuals from M3SC0 (the population before selection) and from M3SC2 (the population after two cycles of selection). A total number of 101 alleles were detected across 24 loci in the 15 lines, whereas 83 alleles were found in the nine M3S progenitors. Among the latter 83 alleles 31 were unique, i.e. found only in one of the progenitors. Mean genetic distance among nine M3S progenitors was 0.61 indicating a broad genetic base of the M3S population. High mean genetic distance was found between M3S progenitors and BSSS lines (0.69) and M3S progenitors and Lancaster lines (0.71). This indicates that the M3S population represents germplasm source unrelated to both the BSSS and Lancaster germplasm. Mean genetic distance between the M3S population and BSSS as well as Lancaster lines slightly decreased after two cycles of recurrent selection suggesting the need of introducing testers from both groups in future selection in the M3S population in order to maintain heterotic complementarity of the M3S population to these groups. A test of selective neutrality identified several non-neutral loci in the population whose allele frequency changes from the C0 to the C2 cannot be explained by genetic drift. The majority of non-neutral alleles, whose frequency increased after two cycles of selection, were present at least in one line from the BSSS or Lancaster heterotic group.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA