Naslov
Increase of bactericidal activity of thymol by EDTA on resistant Gram-negative bacteria

Autori
Bošnjak, Blanka ; Daraboš, Dina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CESAR 2006 / - , 2006

Skup
CESAR 2006-Central European Symposium on Antmicrobial Resistance

Mjesto i datum
Vysoké Tatry, Slovačka, 18.06.2006. - 21.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
thymol; multiple resisatnce; bacteria

Sažetak
Thymol, as hydrophobic phenol-type natural compound has a wide spectrum of antimicrobial activity with MICs below 0.05 % (m/V). Its antimicrobial efficacy could be increased by combining with EDTA, which enhances the permeability of the cell wall of Gram-negative bacteria. The purpose of this study was to determine antimicrobial activity of thymol (2.5, 25 and 50 mg/mL) in combination with Na2EDTA (0.1, 0.5 and 7 mM) against 13 species of clinical isolates of Gram-negative bacteria by cylinder plate assay (P. aeruginosa, P. vulgaris, P. mirabilis, E. coli, E. coli ESBL+, Kl. pneumoniae, Kl. oxytoca, Salmonella spp., Ewdardsiella spp., S. marcescens, Sh. boydii, Sh. flexnerii and V. mimicus). The most effective was combination of 7 mM of EDTA and 25 mg/mL of thymol which significantly increased the bactericidal activity in all tested species, determinate as inhibition zones of growth. The second most effective combination was 0.5 mM od EDTA with 25 mg/mL which were effective against 77% of bacterial species testes. P. aeruginosa was the most resistant against thymol alone, or combination of thymol and EDTA. In time-kill study we used E. coli ESBL+ and P. aeruginosa as models for predicting of bactericidal activity of thymol and combination of thymol and EDTA. During 30 minutes of time-kill study, thymol 50 mg/mL with 5mM EDTA significantly decreased the number of bacterial cells in comparison with thymol alone against P. aeruginosa. Using E. coli, the combination of 0.125 mg/mL of thymol and 5 mM od EDTA killed all bacteria in 30 minutes, while thymol alone killed 70% of inoculum. EDTA potentiates the bactericidal action of thymol, and theirs combination could be used as disinfectants.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

Napomena
Ovaj rad je prezenatcija znanstvenih podataka studentskog rada nagrađenog Dekanovom nagradom u rujnu 2006. godine. Mentori ovog radu su prof.dr.sc. Stjepan Pepepljnjak i dr.sc. Ivan Kosalec

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