Naslov
Frequency of TNF-alpha +489G/A polymorphism in patients with chronic obstructive pulmonary disease

Autori
Malić, Ana ; Smoljanović, Jelena ; Čepelak, Ivana ; Rumora, Lada ; Grdić, Marija ; Popović-Grle, Sanja ; Barišić, Karmela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstract Congress of the Croatian Society of Biochemistry and Molecular Biology on the occasion of the 30th Anniversary with international participation / Kovarik, Zrinka - Zagreb : Hrvatsko društvo za biokemiju i molekularnu biologiju (HDBMB), 2006, 114-114

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology on the occasion of the 30th Anniversary with international participation

Mjesto i datum
Vodice, Hrvatska, 03.10.2006. - 07.10.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
TNF-alpha; Chronic obstructive pulmonary disease; Polymorphism

Sažetak
Tumor necrosis factor (TNF)– alpha, pro-inflammatory cytokine, plays an important role in inflammatory processes. Several biallelic polymorphisms of the gene coding for TNF-alpha (located on chromosome 6 in the class III region of the major histocompatibility complex) are known. Some of them have been reported to be associated with chronic inflammatory diseases. Chronic obstructive pulmonary disease (COPD) is characterised by enhanced level of TNF-alpha in sputum and in circulation indicating that this cytokine is involved in both the local and systematic inflammation present in COPD. Analysis of possible TNF-alpha gene polymorphisms in COPD could be related to a higher susceptibility to develop this disabling pathological condition. We analysed TNF-alpha gene polymorphism at position +489G/A located in the first intron of the gene which has been reported to be associated with COPD in Dutch population. Polymorphism at position +489G/A of TNF-alpha gene was examined in 31 COPD patients and in 51 healthy volunteers by PCR-RFLP procedure. We found following genotype frequencies in the group of COPD patients: AG 0.39, GG 0.61, and AA 0.00 ; and in the control group: AG 0.53, GG 0.45, and AA 0.02. There were no differences on the allele and genotype frequency between the two groups (P=0, 299). We could not find a significant link between the +489G/A polymorphism of TNF-alpha gene and COPD in Croatian population.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA