Naslov
Caprine alpha(s1)-casein polymorphism: Characterisation of A, B, E and F variants by means of various biochemical and molecular techniques

Autori
Feligini, Maria ; Frati, Silvia ; Čubrić Čurik, Vlatka ; Brambilla, Ada ; Parma, Pietro ; Čurik, Ino ; Greppi, Gian Franco ; Enne, Giuseppe

Izvornik
Food Technology and Biotechnology (1330-9862) 43 (2005), 2; 123-132

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
capra hircus; as1-casein; electrophoresis; RP-HPLC; real-time PCR

Sažetak
Considering a wide interest for the characterisation of caprine as1-casein variants and a large number of differently equipped laboratories, the objective of this study was to analyse and compare characteristics of caprine as1-casein variants by means of various biochemical and molecular techniques. The most frequent caprine as1-casein variants (A, B, E and F) were characterized by employing electrophoretic protein separation analyses (capillary zone electrophoresis, isoelectric focusing, and sodium dodecylsulfate polyacrylamide gel electrophoresis), chromatographic analysis (reversed phase-high performance liquid chromatography) as well as DNA analyses (ASA and real-time polymerase chain reaction approach). Further, we stressed weak and strong points for each method applied and provided information for the optimal and complementary use of those methods with respect to time, resolution and costs.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA