Naslov
Decresed expression of bone morphogenetic proteins 2 and 6 in the peripheral blood mononuclear cells of patients with spondyloarhritis

Autori
Jajić, Zrinka ; Kovačić, Nataša ; Grubišić, Fran ; Topić, Iva ; Grčević, Danka ; Marušić, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical and Experimental Rheunatology / - Gent : Clinical and Experimental Rheumatology, 2006, 466-466

Skup
5th International Congress of Spondyloathropaties

Mjesto i datum
Gent, Belgija, 12.10.2006. - 14.10.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
BMPs ; expression ; spondyloarhritis

Sažetak
Bone morphogenetic proteins (BMPs), members of the transforming growth factor-superfamily, originally identified by their bone-inducing activity, have a crucial role in skeletal and joint morphogenesis and metabolism. In spondyloarthtis (SpA), inflammation and excessive reparative responses may lead to inappropriate cartilage and bone formation causing joint ankylosis that thereby contributes to loss of joint function. As a multifunctional cytokines expressed by many different cell types, BMPs may have a role in the interplay between mesenchymal stem cells, synovial fibroblasts, articular chondrocytes, and other inflammatory cells involved in the pathophysiology of SA. It has been already shown that different BMPs and their receptors are expressed by cell populations isolated from various articular tissues, including cartilage, synovial membrane and subchondral bone. The aim of our study was to test changes in the expression pattern of certain bone morphogenetic proteins in peripheral blood mononuclear cells (PBMCs) of patients with SpA, as a group of chronic inflammatory “ remodeling” joint disorders. Blood samples were collected from SpA patients divided into two groups, with ankylosing spondylitis (AS ; n = 10) and psoriatic arthritis (PA ; n = 13), and healthy volunteers (n = 4) after the informed consent. PBMCs were separated by Ficoll-Paque gradient centrifugation. RNA was extracted from PBMC, converted to cDNA and amplified by quantitative PCR, using TaqMan assays for BMP-2 and BMP-6. PCR reactions were conducted in an ABI Prism 7000 Sequence Detection System (Applied Biosystems), in duplicates, and expressed as the relative amounts of RNA (mean ± ; SD) for target genes normalized to GAPDH (endogenous control) using the standard curve method. We found decrease in the expression of BMP-2 (1.41 ± ; 0.67 in AS and 1.01 ± ; 0.33 in PA samples vs. 5.20 ± ; 2.02 in controls of mRNA relative quantity, p < 0.05) and BMP-6 (0.21 ± ; 0.03 in AS and 0.15 ± ; 0.01 in PA samples vs. 0.85 ± ; 0.25 in controls of mRNA relative quantity, p < 0.05) in SpA patients compared with control samples. Our preliminary results indicate that SpA patients have the decreased expression of BMP-2 and BMP-6 in PBMCs, which may indicate the systemic affection of inflammatory and progenitor cells in SpA and, upon their homing to the affected joints, possible influence on the reparative processes in cartilage and bone cells. Our further aim is to test the systemic changes in the expression of BMPs, their receptors, signaling molecules and antagonists in a larger number of patients with different chronic joint diseases and correlate them with the type and severity of the disease.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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