Naslov
Influence of interleukin-1alpha and tumor necrosis factor-alpha production on corneal graft survival

Autori
Bosnar, Damir ; Dekaris, Iva ; Gabrić, Nikica ; Markotić, Alemka ; Lazić, Ratimir ; Spoljarić, N.

Izvornik
Croatian Medical Journal (0353-9504) 47 (2006), 1; 59-66

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cytokines; IL1-a; graft rejection; TNF-1a; cornea

Sažetak
Aim To determine pro-inflammatory cytokine secretion from human corneas with different pathology and to establish whether cytokine profile influences corneal graft outcome. Method Proinflammatory cytokine (interleukin [IL]-1&#945; and tumor necrosis factor [TNF]-&#945; ) secretion was measured after cultivation of 47 corneas collected from corneal graft recipients suffering from different corneal diseases. Non-inflammatory corneal diseases were keratoconus (n = 8), keratoglobus (n = 2), bullous keratopathy (n = 11), and Groenouw stromal dystrophy type II (n = 2), whereas inflammatory included vascularized corneal scar (n = 14), rejected graft (n = 6), and corneal ulcer (n = 4). Corneas were cultivated at 37°C for 24 hours and frozen until cytokine detection was measured by immunoassay. Donor corneas unsuitable for transplantation were used as controls (n = 7). Corneal graft recipients were followed at least 18 months and rejection rate was calculated for each group. Results The median concentration of IL-1&#945; secreted from corneas with non-inflammatory diseases was 2.47 pg/mm3 (range, 0.13-9.95 pg/mm3). In inflammatory corneal diseases, IL-1&#945; concentration was significantly higher (median, 5.92 pg/mm3 ; range, 0.48-12.68 pg/mm3 ; P = 0.005). IL-1&#945; production in controls (median, 0.63 pg/mm3 ; range, 0.36-1.29 pg/mm3) was significantly lower than in inflammatory corneal diseases (P < 0.001) and non-inflammatory diseases (P = 0.008). Low level of TNF-&#945; was detected only in 5 cases of vascularized corneal scars, 3 cases of bullous keratopathy, and 3 cases of graft rejection. Rejection rate was significantly higher in inflammatory than in non-inflammatory group (46% vs <10%, respectively, P = 0.008). IL-1&#945; and TNF-&#945; were absent from all patient&#8217; s sera, confirming its local intra-ocular production. Conclusion Increased production of IL-1&#945; by corneal recipients with inflammatory diseases suggests its role in corneal graft rejection in humans.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA