Naslov
Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pratense L.) changes caused by natural selection

Autori
Grljušić, Sonja ; Bolarić, Snježana ; Popović, Svetislav ; Čupić, Tihomir ; Tucak, Marijana ; Kozumplik, Vinko

Izvornik
Periodicum biologorum (0031-5362) 110 (2008), 3; 237-242

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Trifolium pratense L.; morphological traits; RAPD markers

Sažetak
Accurate assessment of the levels and patterns of genetic variability and identification of differences for response to natural selection can be invaluable in red clover breeding for diverse applications. The study was conducted to compare the efficiency of morphological and random amplified polymorphic DNA (RAPD) markers in evaluation of changes in red clover (Trifolium pratense L.) cultivars caused by natural selection. Three red clover cultivars and their selections, which had survived for three years growing in an upland region, provided the basic material. Morphological and RAPD data were analysed by NTSYS-pc software. Number of markers detected, values and ranges of distances calculated and 2D Principal Coordinate Analyses (2D PCoA) based on both marker techniques were compared. Although both marker methods indicated the differences among cultivars and selections, parameters differed in values. The number of loci detected by RAPD markers was much higher than that determined with morphological traits. The values and ranges of morphological distances were higher and wider than those of molecular distances. The eigenvectors of 2D PCoA based on morphological data explained more of the total variation observed in the whole data set. Each of the data sets has its own strengths and constraints. For reasonably accurate estimates of genetic variability and its changes under natural selection, adequate attention has to be devoted to both marker methods.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA