Naslov
Apoptosis of Caco-2 cells caused by ADAP in relation to expression of p53, c-ras, c-mos and caspase 3

Autori
Marczi, Saška ; Glavaš-Obrovac, Ljubica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 31st FEBS Congress / Perham, Richard ; Apweiler, Rolf et. al. - Istanbul : Wiley-Blackwell, 2006

Skup
31st FEBS Congress, Molecules in Health and Disease

Mjesto i datum
Istanbul, Turska, 24.06.2006. - 29.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
apoptosis; DNA intercalation; Caco-2 cells

Sažetak
4-Methyl-2, 7-diamino-5, 10-diphenyl-4, 9-diazapyrenium hydrogensulfate (ADAP) is a DNA and RNA intercalator. Cytotoxicity of ADAP was analysed using the MTT assay. ADAP caused strong growth inhibitory effects on human colon carcinoma (Caco-2) cells in concentrations 1 and 10 μ M, in comparison to human normal cells WI38. Apoptotic characteristics of treated Caco-2 cells (1 μ M ADAP for 1 hour) were observed using annexin-V-fluorescein. Immunoband depletion assay, performed with 0, 1 mM ADAP on Caco-2 cells, showed that ADAP is not a topoisomeraseII poison. The mRNA expression of the genes p53, c-Ki-ras, c-N-ras, c-H-ras, c-mos and caspase 3, in Caco-2 cells treated with 1 μ M ADAP, was examined by RT-PCR. The expression of all analysed genes, except for c-H-ras and p53, was dependent on the incubation time (3, 12 and 24 hours). Transcripts for c-H-ras were not detected in treated Caco-2 cells when compared to control nontreated cells, and amount of the p53 mRNA in treated cells were similar to control cells. Conclusion: Apoptosis of the Caco-2 cells treated with ADAP was a result of the mode of action different than inhibiting the topoisomeraseIIα enzyme. ADAP caused the increased expression of c-Ki-ras, c-N-ras, c-mos and caspase 3 gene products, which pointed out the possible role of these genes in apoptotic cell death of the treated Caco-2 cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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