Naslov
P16INK4a expression in human papilloma virus related cervical lesions

Autori
Krivak Bolanča I., Šentija K.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Cytopathology (Suppl.2) / - : Wiley-Blackwell, 2005, 70-71

Skup
31st European Congress of Cytology

Mjesto i datum
Pariz, Francuska, 02.10.2005. - 05.10.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
human papilloma virus; cervical dysplasia; molecular biomarkers

Sažetak
BACKGROUND: Molecular biomarkers for dysplastic cells are belived to solve current problems associated with specificity and sensitivity of Pap test. Based on the understanding of the molecular pathogenesis of cervical cancer and the key role of the human papilloma virus oncogenes, the cyclin-dependent kinase inhibitor p16INK4a emerged as an biomarker to identify HPV-infected cells in histological and cytological slides. OBJECTIVES:The probability of progression of cervical precancerous lesion is considered greater with the persistence of high-risk human papillomavirus (HR HPV) infection and age. The present study evaluated the expression of p16INK4a in cervicovaginal smears with low grade squamous intraepitelial lesion (LG-SIL), in high grade (HG SIL) lesion and in squamous carcinoma all with recognized and documented high risk HPV infection by DiGene method, suggesting that immunocytochemical staining of p16INK4a is convenient method for triaging dysplastic cells infected with HR HPV. METHODS: Conventional cervicovaginal cytology specimens from 23 patients were taken and analyzed. Simultaneously, sixteen (16) slides were taken for immunostaining with primary antibody p16INK4a and seven were decolorized in 0, 2%-0, 5% diluted solution of HCl , rinced with distilled water and immunocytochemically stained. High risk HPV infection was documented in all patients.RESULTS: Positive p16INK4a staining occured in 28, 57% (4/14), 100%(7/7)and 100%(2/2) in LG-SIL, HG-SIL and squamous carcinoma, respectively. Reaction of immunostaining was severe in decolorized slides as well as in those taken simultaneously with conventional Pap smear. Staining for p16INK4a in HG SIL and in carcinoma showed more intense than in LG SIL.CONCLUSION: Because high risk type HPV was identified in all smears with high-grade dysplasia and carcinoma with strong positive p16INK4a staining, the association of p16INK4a overexpression with HR-HPV infection was confirmed. LG SIL with HR HPV infection did not show expected positivism for p16INK4a. These may means that these patients are not in danger of developing cancer inspite of high risk HPV infection, and that another trigger is necessary so cells may switch into a process of carcinogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA