Naslov
Mitochondrial Dysfunction enhances Gal4 dependent Transcription

Autori
Jeličić, Branka ; Traven, Ana ; Filić, Vedrana ; Sopta, Mary

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EMBO/HHMI central European Scientist Meeting Conference Proceedings : Abstracts / Ugarković, Đurđica - Zagreb, 2006, 60-60

Skup
EMBO/HHMI central European Scientist Meeting

Mjesto i datum
Cavtat, Hrvatska, 15.06.2006. - 17.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Gal4; transcription

Sažetak
Mitochondrial dysfunction has been shown to elicit broad effects on nuclear gene expression. We show here that transcription dependent on the prototypical acidic activator Gal4 is responsive to mitochondrial dysfunction. In cells with no mitochondrial DNA, Gal4-dependent gene expression is elevated. A minimal Gal4 activator containing the DNA binding and activation domain is sufficient for this response. Transcription dependent on a fusion of Gal4 to a heterologous DNA binding domain is similarly elevated in a mitochondrial mutant. Analysis of different Gal4-dependent promoters and gel mobility shift assays suggest that the effect of mitochondrial dysfunction on Gal4 activity is related to increased DNA binding to the cognate Gal4 element. Given that fermentation is the only means to obtain energy in respiratory deficient cells, it is possible that higher Gal4 activity in cells with dysfunctional mitochondria works to promote more efficient fermentation of galactose.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA