Naslov
Rapid loss of Oct-4 expression and pluripotency in cultured embryonic stem cells derived from Non-obese diabetic mice

Autori
Korolija, Marina ; Popović Hadžija, Marijana ; Hadžija, Mirko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
EMBO / HHMI Central European Scientists Meeting

Mjesto i datum
Dubrovnik, Hrvatska, 15.06.2006. - 17.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Oct-4; NOD; embryonic stem cells

Sažetak
The POU transcription factor Oct-4 has been characterized as a regulator of ES cell pluripotency. Therefore we have tested the ability of NOD mice ES cells to remain pluripotent in primary culture by monitoring Oct-4 expression over a five day period. Although the ES cells have been cultured on feeder layer prepared from primary embryonic fibroblasts, in culture media supplemented with leukemia inhibitory factor (in order to prevent differentiation), Oct-4 expression has been detectable by RT-PCR only during first three days of culturing. In contrast, ES cells derived from blastocysts of various mouse strains (Balb/C, C57Bl/GoZg and C3H) grown in identical culture conditions have retained Oct-4 expression throughout entire five day period. Consistent with that observation, morphology of NOD ES cells has confirmed loss of pluripotency by the fourth day of culturing (appearance of giant trophoblast cells and fibroblast-like cells), while ES cells of other tested strains have retained typical pluripotent, undifferentiated phenotype (small cells with large nuclei and minimal cytoplasm). Based on obtained results, we have concluded that early loss of Oct-4 expression and pluripotency (i.e. onset of differentiation) in cultured NOD mice blastocysts could be a limiting factor in establishment of ES cell line.

Izvorni jezik
Engleski

POVEZANOST RADA