Naslov
Effect of allergen dose on airway inflammation and enhancment of airway hyperresponsiveness

Autori
Bošnjak, Berislav ; Ferenčić, Željko ; Đurić, Koraljka ; Brajša, Karmen ; Glojnarić, Ines ; Marković, Stribor ; Antolović, Roberto ; Hrvačić, Boška

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book of 2006 NIAID, National Institute of Health, Research Conference, Opatija, Croatia, June 26-30, 2006 / ... - Rijeka : ..., 2006

Skup
2006 NIAID, National Institute of Health, Research Conference,

Mjesto i datum
Opatija, Hrvatska, 26.06.2006. - 30.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Airway inflammation (AI); hyperresponsiveness (AHR); asthma

Sažetak
Airway inflammation (AI) and hyperresponsiveness (AHR) are two asthma hallmarks. Allergen inhalation in sensitized patients induces late-phase responses (LPR) characterized by accumulation of inflammatory cells that further enhances AHR. Therefore, allergen-induced LPR was proposed as a model for studying the pathophysiological processes occurring in asthma. As clinical investigations suggest that the dose of inhaled allergen directly influences the appearance and the severity of LPR, we intranasally challenged ovalbumin (OVA) sensitized mice with increasing OVA doses (10, 25 or 100  g OVA/mice) in order to provide new insights into the complex mechanisms by which AI enhances AHR. AHR to methacholine, measured non-invasively at its peak 24h after challenge, was increased only in mice challenged with largest OVA dose (100  g), indicating dependence of AHR on the antigen dose used for challenge. On contrary to AHR enhancement, eosinophil accumulation in bronchoalveolar lavage (BALF) and lungs 24h after challenge was almost insensitive to the OVA dose used for challenge. Interestingly, accumulation of neutrophils in BALF seems to be correlated to the OVA dose, indicating that neutrophils could play important role in AHR enhancement. However, neutrophil accumulation and AHR enhancement could be two simultaneous, but independent phenomena.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA