Pregled bibliografske jedinice broj: 272958
Molecular epidemiology of giardiasis: lessons from multilocus genotyping of human and animal isolates
Molecular epidemiology of giardiasis: lessons from multilocus genotyping of human and animal isolates // 11th International Congress of Parasitology(ICOPA XI)
Glasgow, Ujedinjeno Kraljevstvo, 2006. (plenarno, nije recenziran, sažetak, znanstveni)
CROSBI ID: 272958 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Molecular epidemiology of giardiasis: lessons from multilocus genotyping of human and animal isolates
Autori
Cacciò ; ; , Simone Mario ; Beck, Relja ; Lalle Marco ; Pozio, Edoardo
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
11th International Congress of Parasitology(ICOPA XI)
Mjesto i datum
Glasgow, Ujedinjeno Kraljevstvo, 06.08.2006. - 11.08.2006
Vrsta sudjelovanja
Plenarno
Vrsta recenzije
Nije recenziran
Ključne riječi
Giardia duodenalis; multilocus genotyping
Sažetak
The epidemiology of human giardiasis is complex and the relative contribution of antroponotic and zoonotic transmission is still unclear. The occurrence of zoonotic genotypes of Giardia duodenalis in livestock, pets and wildlife is well established, but conclusive evidences of transmission to humans, either directly or indirectly through water or food contamination, are still lacking. Furthermore, molecular investigations have been frequently based on the analysis of a single genetic marker, an approach that may result in an incorrect identification of species/genotypes. In this study, we investigated how reliable is the identification of genotypes as a function of the genetic markers and/or the origin of the parasite (host). The markers chosen were fragments of the genes encoding for the small subunit ribosomal DNA, b-giardin, glutamate dehydrogenase, triose phosphate isomerase and ORF-C4, which were PCR amplified and sequenced. The G. duodenalis isolates were faecal samples from humans, pets, livestock, rodents and wildlife (n=150). A consistent and reliable assignment of genotypes was observed for the human isolates using the 5 markers, and multilocus genotypes were identified for both assemblages A and B. On the other hand, the characterization of animal isolates yielded a different and more complex picture. In particular, the genotype assignment of Giardia isolates from dogs was problematic, as different markers gave different results in about 30% of cases. A similar result was observed for isolates collected from other hosts, albeit at a lower frequency (5-10%). The practical implications of these results, as well as the possible mechanisms that are responsible for, will be discussed.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Veterinarska medicina
