Naslov
Genetic diversity of Bradyrhizobium japonicum field population revaled by RAPD fingerprinting

Autori
Sikora, Sanja ; Redžepović, Sulejman ; Pejić, Ivan ; Kozumplik, Vinko

Izvornik
Journal of applied microbiology (1364-5072) 82 (1997), 4; 527-531

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
nitrogen fixation; Bradyrhizobium japonicum; indigenous strains; RAPD fingerprinting

Sažetak
RAPD fingerprinting was used for strain identification and the assesment of genetic diversity within a field population of Bradyrhizobium japonicum. Total genomic DNAs from 13 field isolates and two inoculant strains were amplified using six different 10-mer primers. Different and informative band patterns were obtained for all strains analysed. Cluster analysis unexpectedly revealed that none of the field isolates was identical to inoculant strains which were regurarly used for soybean inoculation. Among field isolates two highly divergent groups were determined. The results indicate that RAPD is very discriminative and efficient method for differentiating and studying genetic diversity of B. japonicum strains.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA