Naslov
Pre-transplant and post-transplant sCD30 levels, tnf-alpha and sIL-2r as risk factors for renal allograft rejection

Autori
Humar, Ines ; Puretić, Zvonimir ; Goreta, Nedjeljka ; Puc, Maja ; Mihaljević, Željko ; Pasini, Josip ; Brkljačić-Kerhin, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 1st World Transplant Congress (WTC 2006) / - , 2006

Skup
World Transplant Congress (1 ; 2006)

Mjesto i datum
Boston (MA), Sjedinjene Američke Države, 22.07.2006. - 27.07.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Kidney transplantation; monitoring; rejection; T cell activation

Sažetak
Allograft rejection is associated with T cell activation. T cell activation leads to secretion and elevated serum soluble IL-2 receptor (sIL-2R) levels. The CD30 molecule, a member of the tumor necrosis factor receptor family, is preferentially expressed on human CD4+ and CD8+ T cells. A soluble form of CD30 (sCD30) is released into the blood following immune activation. In the present study we evaluated whether the presence of serum sCD30 correlated with clinical events following renal transplantation (Tx). The pre and post Tx sera (day 4, - 8, - 14 and - 30) of 127 primary recipients of a deceased donor renal allograft were tested for the presence of sCD30 (U/ml), sIL-2R (U/ml), TNF- (pg/ml) using an ELISA kits. For statistical difference Cox regression was used. Immunological risk factors (PRA 12, 60% and HLA MM 2, 8 1, 1) had no impact. The results were correlated with graft rejection, survival and degree of recipient sensitization. The presence of HLA Ab is thought to be an immunological risk factor in renal transplantation. Due to small number recipients with PRA, where was not association with sCD30 and graft rejection. High sCD30 was associated with poor graft survival and rejection. The pre Tx sCD30 for recipients experiencing rejections was greater than the sCD30 for non-rejecting recipients (370 93, 6 vs. 165 32, 97 U/ml, P=0.002). Post Tx sCD30 was significant on day 8 at the level of significance P=0, 028 (rejection vs. non-rejection). Differences of sCD30 were present with no significance on day 4, 14 and 30. Recipients undergoing severe rejection episodes had higher overall serum levels of sIL-2R on day 4 (449 115, 46 U/ml vs. 240, 88 53, 45 U/ml) as compared with recipients who had stable renal function and no episodes of rejection (P= 0.027). Comparison of sIL-2R ratios (posttransplant sIL-2R level/pre-transplant sIL-2R level) revealed that ratios of 0.6 or higher were more frequently seen in patients who subsequently underwent severe rejection episodes. TNF- was significantly different pre Tx (P=0, 012) and on day 8 (P=0.0307). Independent of classical immunological risk factors, these data suggest that high level of sCD30 and TNF- (members of the tumor necrosis factor family) reflect an immunological risk for rejection while sIL-2R maybe predictive of severe rejection episodes. Moreover, serial monitoring may identify changes that correlate with post Tx clinical events.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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