Naslov
Preliminary results of surgical equine embryo transfer after open pulled straw vitrification

Autori
Duchamp, Guy ; Guignot, Florance ; Grizelj, Juraj ; Vidament, Marianne ; Mermillod, Pascal

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Reproduction, fertility and development / - Orlando (FL) : International Embryo Transfer Society, 2006, 154-154

Skup
32nd Annual Conference of the International Embryo Transfer Society

Mjesto i datum
Orlando (FL), Sjedinjene Američke Države, 08.01.2006. - 10.01.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
vitrification; equine; embryo; surgical; transfer

Sažetak
In equine species, embryo cryopreservation is not as widely developed as in some other species. Slow freezing has been applied to equine embryos but with relatively low success rates. This higher sensitivity to conventional freezing procedures may be explained by the presence of a capsule surrounding the equine embryo that may impair cryoprotectant penetration. Recently, good in vitro embryo survival rate was obtained after OPS vitrification (Moussa et al, Theriogenology, 2005, in press). The aim of the present study was to evaluate in vivo survival of vitrified embryos five days after surgical transfer into Welsh pony mares. Morulae (M), early blastocysts (EB) and blastocysts (B) ranging from 140 to 320 µ ; m in diameter were collected (n=20) in a Ringer lactate solution on Day 6.75 after ovulation. Before vitrification, embryos were assessed morphologically and their size was measured (McKinnon AO and Squires EL, J Am Vet Med Assoc, 1988, 192:401-406). Then, embryos were vitrified using the OPS method described by Berthelot et al. (Reprod Nutr Dev 2001, 41:267-72). Briefly, embryos were twice washed in HepesTCM199 + 20% newborn calf serum(NBCS) for 1 min, equilibrated in Hepes TCM199 + 20% NBCS with 7.5% DMSO + 7.5% EG for 3 min and with 18% DMSO + 18% EG + 0.4 M sucrose for 45 sec. One embryo was then loaded per straw. For transfer, four straws were quickly thawed (5 sec in air) and the narrow end of the straw containing the embryo was immersed in Hepes TCM199 + 20% NBCS + PBS + 0.2 M sucrose. Five to 8 min after thawing, four embryos were surgically transferred into the cranial portion of the uterine horn in each of five pony mare recipients. Five days after transfer, embryos recovered by transcervical flushing of the uterus were classified as viable if morphology was normal, no dark inner cells were present, the capsule was intact and diameter was at least 1000µ ; m. The results are shown in the table. One recipient of vitrified embryos had an endometritis and no embryo was recovered. From the four other recipients, nine embryos were recovered out of 16 (56%) embryos transferred, seven of which were viable (44%). The results of the present preliminary study demonstrating survival of equine embryos transferred after OPS vitrification is very encouraging. However, the results should be confirmed by birth of foals after transfer of OPS vitrified embryos to recipients.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija

POVEZANOST RADA