Naslov
Genetic analysis of Staphylococcus aureus isolates from Croatia

Autori
Budimir, Ana ; Bošnjak, Zrinka ; Deurenberg, Ruud H. ; Stobberingh, Ellen E. ; Kalenić, Smilja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12th International Symposium on Staphylococci and Staphylococcal infections : abstracts / Peters, Georg ; Vaudeaux, Pierre ; Verbrugh, Henri - Maastricht : Congress Care, 2006, 114-114

Skup
International Symposium on Staphylococci and Staphylococcal infections (12 ; 2006)

Mjesto i datum
Maastricht, Nizozemska, 03.09.2006. - 06.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MRSA; PFGE; PVL; genotypes; CA MRSA

Sažetak
Background Methicillin-resistant Staphylococcus aureus (MRSA) clones are disseminating worldwide. This study investigated the susceptibility patterns of S. aureus isolates, the genetic background of MRSA strains and the prevalence of Panton-Valentine leukocidin (PVL) among MRSA and methicillin-sensitive S. aureus (MSSA) isolates from Croatia. Methods Successive S. aureus isolates (n=1815) were collected from 27 Croatian laboratories in 20 cities during a three-month surveillance period (October-December 2004). Susceptibility testing for 18 antimicrobial agents was performed with disk-diffusion and broth micro-dilution according to CLSI and BSAC (mupirocine and fucidic acid) guidelines. Methicillin resistance was confirmed by mecA PCR. SCCmec typing was performed with a multiplex PCR and pulsed-field gel electrophoresis (PFGE) was performed after digestion of chromosomal DNA with SmaI. PVL was detected with PCR. Results A total of 259 strains (14%) were identified as MRSA, 96% of which were multi-resistant. All MRSA isolates were susceptible to vancomycin and 0.22% was resistant to linezolid. The majority of the MRSA strains harboured SCCmec type I (88%) and 2% type II, 2% type III and 4% type IV. None of the isolates harboured SCCmec type V and 4% of the isolates were not-typeable. PVL was detected in 3 isolates and harboured SCCmec type IV. PFGE typing was performed on 174 strains so far and revealed five major similarity groups: D, E, H, J, and N with 10%, 6%, 12%, 28%, and 6% of the isolates respectively. PFGE analysis also showed 10 minor groups containing between 1% and 3% of the isolates, and 17% were solitary isolates. Conclusion PFGE analyses showed five major similarity groups and the predominance of SCCmec type I among MRSA isolates from Croatia. PVL was detected in 3 MRSA isolates, all harbouring SCCmec type IV. The MRSA stains will be further characterized using MLST and spa typing.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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