Naslov
Activation of phosphoinositide 3-kinase C2beta in the nuclear matrix during compensatory liver growth.

Autori
Sinđić, Aleksandra ; Crljen, Vladiana ; Matković, Katarina ; Lukinović-Škudar, Vesna ; Višnjić, Dora ; Banfić, Hrvoje

Izvornik
Advances in enzyme regulation (0065-2571) 46 (2006), 1; 280-287

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
PI 3-K-C2beta ; nuclei ; matrix ; rat ; liver ; growth

Sažetak
In the nuclear matrix harvested 20 h after partial hepatectomy, an increase in immunoprecipitable PI3K-C2beta activity is observed, which is sensitive to wortmannin (10 Mm) and shows strong preference for PtdIns over PtdIns(4)P as a substrate. On western blots PI3K-C2beta revealed a single immunoreactive band of 180 kD, whereas 20 h after partial hepatectomy gel shift of 18kDa was noticed in the nuclear matrix, suggesting that observed activation of enzyme is achieved by proteolysis. As it is know that PI3K-C2alpha is associated with nuclear speckles [Didichenko SA, Thelen M. Phosphatidylinositol 3-kinase C2alpha contains a nuclear localization sequence and associates with nuclear speckles. J Biol Chem 2001 ; 276:48135-42.], the data presented in this report show that in the nuclear matrix PI3K-C2beta is activated during the compensatory liver growth, which clearly demonstrates that different class II PI3K enzymes have different subnuclear localization and therefore might have different intranuclear functions.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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