Naslov
BMP-2, BMP-4, AND BMP-6 Inhibit the Differentiation of Leukemic NB-4 Cell Line Induced by All-trans-retinoic Acid Treatmet

Autori
Topić, Iva ; Ivčević, Sanja ; Kušec, Rajko ; Marušić Vrsalović, Maruška ; Salopek, Daniela ; Marušić, Ana ; Grčević, Danka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th International Conference on Bone Morphogenetic Proteins : Abstract Book / - , 2006

Skup
International Conference on Bone Morphogenetic Proteins (6 ; 2006)

Mjesto i datum
Cavtat, Hrvatska, 11.10.2006. - 15.10.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
NB4 stanična linija; koštani morfogenetski proteini; diferencijacija
(NB4 cell line; bone morphogenetic proteins; differentiation)

Sažetak
Acute promyelocytic leukemia (APL) involves leukemic blasts expansion blocked at the promyelocytic stage and reciprocal translocation t(15 ; 17), which results in the fusion of promyelocytic leukemia (PML) and retinoic-acid receptor-α (RARα ) genes. NB-4 cell differentiation was induced by ATRA (0.5 µ M), with or without BMP-2, BMP-4, or BMP-6 treatment (1, 5 pg/ml), and confirmed by increase in granulocytic antigen CD11b expression on flow-cytometry. Promyelocytic NB-4 line expressed Alk-2, Alk-3, Alk-6, and RII BMP receptors, which made it responsive to BMP treatment. Combined treatment with ATRA and BMP-2, BMP-4, or BMP-6 significantly blocked the promyelocytic differentiation, evidenced as up-regulation of CD11b expression (more than 95% inhibition of CD11b expression by ATRA and BMP-2, BMP-4, or BMP-6 (1 pg/ml for both) compared to ATRA alone). Combination of ATRA and BMP-2, BMP-4, or BMP-6 had greatest influence on increase in Alk-6 expression, without significant changes in the expression of PML/RARA-oncogene.

Izvorni jezik
Engleski

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