Pregled bibliografske jedinice broj: 268483
THE DEVELOPMENT OF A MOUSE MUTANT FOR STUDYING THE ROLE OF NKG2D IN VIVO
THE DEVELOPMENT OF A MOUSE MUTANT FOR STUDYING THE ROLE OF NKG2D IN VIVO // 1st Joint Meeting of European National Societies of Immunology - 16th European Congress of Immunology / Bernard, Alain ; Kaufmann, Stefan H.E. (ur.).
Pariz: EFIS, 2006. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 268483 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
THE DEVELOPMENT OF A MOUSE MUTANT FOR STUDYING THE ROLE OF NKG2D IN VIVO
Autori
Zafirova, Biljana ; Antulov, Ronald ; Polić, Bojan
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
1st Joint Meeting of European National Societies of Immunology - 16th European Congress of Immunology
/ Bernard, Alain ; Kaufmann, Stefan H.E. - Pariz : EFIS, 2006
Skup
1st Joint Meeting of European National Societies of Immunology - 16th European Congress of Immunology
Mjesto i datum
Pariz, Francuska, 06.09.2006. - 09.09.2006
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
NKG2D; NK cells; Cre/loxP technology; embryonic stem cells
Sažetak
NKG2D is an activating receptor that is involved in the innate and adaptive immune response to various forms of cellular stress (infections, heat shock, etc.) and tumour transformation. In mice, it is expressed on NK, NKT and T (CD8+ activated and memory ab and some gd) cells. NKG2D is a transmembrane type II glycoprotein, encoded by a gene situated within the NK complex of the mouse chromosome 6, which binds different, mostly stress-induced MHC class I-like molecules (MULT-1, H60 and Rae 1 family) on wide variety of cell types. Although the role of NKG2D has been intensively investigated in different models (viral infections, tumour immunosurveillance, autoimmunity, etc.), to address more specific questions on the role of NKG2D in the development, homeostasis and effector functions of the immune system the use of a genetic approach seems to be inevitable. Therefore, we have generated NKG2D knock out mouse strain by targeting of NKG2D in Bruce 4 (C57BL/6) embryonic stem (ES) cells. Following the selection and screening procedure, we have identified several ES cell clones positive for the homologous recombination of the targeting vector. Upon the microinjection of the clones we have obtained several chimeras that have given germ-line transmission of the mutation.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
