Naslov
Expression of BMPs in adult human osteophyte tissue

Autori
Zoričić, Sanja ; Marić, Ivana ; Šantić, Veljko ; Bobinac, Dragica ; Vukičević, Slobodan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Bone / Baron, Roland - New York (NY) : Elsevier, 2001, S109-S109

Skup
1st Joint Meeting of the International Bone and Mineral Society and the European Calcified Tissue Society

Mjesto i datum
Madrid, Španjolska, 05.06.2001. - 10.06.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bone morphogenetic proteins; osteophytes; humans; immunohistochemistry

Sažetak
Osteoarthrisis is characterized by focal cartilage destruction and marked formation of osteophytes. This study was performed on a series of 25 osteophytes removed from the knee and hip joint during joint replacement surgery. Ostophyteswere recognized as a small overhanging lips located usually at the margin of articular surface. Specimens were fixed in 4% PFA, undecalcified embedded in MMA and cut serially at 200 mm intervals on 4 mm thick slices. The mechanism of osteophyte formation was examined histologically and immunohistochemically, utilizing specific antibodies for bone morphogenetic proteins (BMPs). Histological analysis revealed loose fibrous tissue on osteophyte's surface and transitional zone of fibrocartilage beneath it. The zone of hyaline cartilage faced to fibrocartilage and showed typical morphology of articular cartilage such as arrangement of cartilage zones and cartilage matrix staining pattern by toluidine blue and safranine O. Osteophytes hyaline cartilage have not contained tide mark line but it had contained large chondrocyte clusters. On the eroded cartilage surface invaded by blood vessels, a mass of osteoclastic and mononuclear cells were visible as well as numerous deposited osteoblasts and osteocytes embedded in osteoid. Fibrous tissue close to bone trabeculae was observed either on surface of osteophytes or within areas of active bone remodeling in osteophytes. BMPs were immunohistochemically observed in: 1) hypertrophic chondrocytes (BMP-3, and – 7), 2) osteoblasts (BMP-2, -3, -5, and – 7), 3) osteocytes (BMP-3, -6, and – 7), 4) bone matrix (BMP-3, -6, and – 7), 4) cartilage matrix (BMP-3), and 5) fibrous tissue matrix (BMP-2, and – 3). In a typical osteophyte the arrangement of all zone followed normal cellular events present in endochondral and/or intramembranous bone formation. The pattern of BMPs in osteophytes tissue suggests that members of the BMP family may have a specific role in formation of osteophytes removed from osteoarthritic joints.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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