Naslov
Study of microsatellite polymorphisms around HLA-B locus in the Croatian population

Autori
Grubić Zorana ; Štingl Katarina ; Žunec Renata ; Čečuk-Jeličić Esma ; Brkljačić-Kerhin Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
20th European Histocompatibility and Immunogenetics Conference

Mjesto i datum
Oslo, Norveška, 08.06.2006. - 11.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HLA-B; miocrosatellites; Croatians

Sažetak
The aim of the present study was to investigate polymorphisms and haplotypes of MICA, D6S2927 and D6S2793 microsatellites and linkage disequlibria (D) between these microsatellite loci and HLA-B in the Croatian population. A total of 179 healthy unrelated Croatians were studied using PCR amplification and electrophoresis on 6% polyacrylamide gel in an automated sequencer (ALFexpress). In total, 6 alleles of D6S2927, 5 alleles of MICA microsatellite locus and 14 alleles of D6S2793 microsatellites were observed. At D6S2927 locus most common allele was D6S2927-4 (23.5%), MICA-5.1 allele was the most frequent (27.6%) at MICA microsatellite, while at D6S2793 locus the most frequent was D6S2793-CA15 (13.1%). Eight HLA-B/D6S2927 haplotypic associations (B*07/D6S2927-4, B*08/D6S2927-3, B*18/D6S2927-3, B*27/D6S2927-1, B*35/D6S2927-5, B*38/D6S2927-4, B*51/D6S2927-32 and B*61/D6S2927-1) showed strong association (p<0.001, D>0.5). Among 88 different HLA-B/MICA haplotypic associations, seven combinations (B*07/MICA-5.1, B*08/MICA-5.1, B*15/MICA-5, B*18/MICA-4, B*27/MICA-4, B*38/MICA-9 and B*51/MICA-6) demonstrated high linkage (D>0.5) with significant p value (p<0.001). Strong association was also observed for 5 HLA-B/D6S2793 haplotypes (B*07/D6S2793-CA17, B*08/D6S2793-CA24, B*137/D6S2793-CA18, B*14/D6S2793-CA14, B*27/D6S2793-CA14). Linkage analysis between tested microsatellites also revealed strong association (D>0.5, p<0.001) for the following combinations: D6S2927-5/MICA-9, D6S2927-3/D6S2793-CA25, D6S2927-4/D6S2793-CA17, D6S2927-3/D6S273-4, D6S2927-3/D6S273-7 and D6S2793-CA26/D6S273-4. These results provide data about region around HLA-B locus which is very attractive because of its contribution to genetic susceptibility for many HLA associated disease and will help in all further HLA-B locus associated disease studies.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA