Naslov
Alkaline Phosphatase Activity in Seawater: The Influence of Reaction Conditions on the Kinetic Parameters of ALP

Autori
Orhanović, Stjepan ; Pavela-Vrančič, Maja

Izvornik
Croatica Chemica Acta (0011-1643) 73 (2000); 819-830

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aquatic enzymes; alkaline phosphatase; kinetic properties; method optimization

Sažetak
Alkaline phosphatase is an extracellular enzyme enabling the utilisation of phosphomonoesters as a source of Pi required for the maintenance of the cellular metabolism. Its catalytic properties have been extensively studied in aquatic environments in an attempt to describe its influence on phosphorous cycling in natural waters. Despite its significance and frequent determination a standard method has not been developed yet. In order to examine the effect of various reaction conditions on the kinetic properties of ALP in seawater the stability of ALP in chloroform-stabilised samples has been studied in addition to the influence of the buffer type, its concentration and pH, the metal-ion content, Pi and incubation time. It could be concluded that the kinetic properties of ALP are significantly influenced by the buffer pH, the concentration of Mg2+ and Zn2+ ions and the incubation period, requiring a precise definition of these reaction parameters in determining a standard method for measurement of ALP activity in water media.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA