Naslov
Inflammatory Cytokines in Lung Lavage Fluid Determined by Bead-Based and ELISA Cytokine Assays

Autori
Kulhankova, Katarina, Hađina, Suzana, Thorne, Peter S.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The Journal of Allergy and Clinical Immunology, Abstract Supplement / - : Mosby, 2006

Skup
American Academy of Allergy, Asthma & Immunology Annual Meeting 2006

Mjesto i datum
Miami Beach (FL), Sjedinjene Američke Države, 03.03.2006. - 07.03.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
multiplex bead-based suspension arrays; ELISA; cytokines

Sažetak
Rationale: Multiplex bead-based suspension arrays enable simultaneously measurement of multiple analytes in a single sample compared to traditional biochemical methods that detect proteins individually. We sought to validate the multiplex suspension array system for analysis of cytokines in murine lung lavage samples. Methods: Murine models of cockroach allergen (Bla-g) and endotoxin-induced lung inflammation were utilized to generate bronchoalveolar lavage (BAL) samples assayed for inflammatory cytokines by multiplex and ELISA methods. Standards with known concentrations of cytokines served for generation of standard curves or as positive controls. In addition, various conditions affecting performance of the multiplex assay were tested. Results: Analysis of BAL fluids yielded quantifiable data with lower levels of detection for multiplex assays as compared to ELISA. Endotoxin inhalation resulted in secretion of inflammatory cytokines/chemokines IL-1, IL-6, TNF-α , IL-12p40, KC, MIP-1α , and RANTES in the airways. Bla-g-exposed animals had detectable IL-5, IL-10, TNF-α in their BAL fluids. We also report the effects of various conditions on performance of the multiplex assay, such as adding bovine serum albumin (BSA) in the BAL samples, blocking wells with BSA, re-reading plates, and measuring cytokine levels in known positive controls. Conclusions: Multiplex bead-based cytokine assays performed in this study yielded quantifiable data and proved to be a valid method to determine cytokine concentrations in murine bronchoalveolar lavage samples. Funded By: NIEHS P30 ES05605

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina

POVEZANOST RADA