Naslov
Endo-deoxyribonuclease from Streptomyces rimosus

Autori
Vukelić, Bojana ; Ritonja, Anka ; Vitale, Ljubinka

Izvornik
Applied microbiology and biotechnology (0175-7598) 43 (1995); 1056-1060

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Endo-deoxyribonuclease ; Streptomyces rimosus ; Isolation ; Characterization

Sažetak
From filtrates of an oxytetracycline-producing culture of Streptomyces rimosus a deoxyribonuclease was purified to homogeneity and determined to be a potent endo-DNase. It is a monomeric, basic protein (Mr≈ 21 000 ; pI≈ 9.5) stable in a broad pH range but unstable to higher temperature.The enzyme has an absolute requirement for Mg^2+ or Mn^2+, and for its full activity requires free SH groups and a low-ionic-strength environment. Its N-terminal primary structure differs from that of other nucleases. The presence of DNase in culture filtrates of the oxytetracycline producer Streptomyces rimosus was reported by Penzikova et al. (1976), but the properties of the enzyme were not studied. An interest in improving the S. rimosus strain and adapting antibiotic production from waste waters, motivated the isolation and characterization of an extracellular nuclease of this microorganism.

Izvorni jezik
Engleski

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