Naslov
Activity of the aidB gene in Salmonella typhimurium

Autori
Perić, Nataša ; Bačun-Družina, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book / Eckl, Peter - Salzburg : European Environmental Mutagen Society, 1998

Skup
28th Annual Meeting

Mjesto i datum
Salzburg, Austrija, 07.09.1998. - 11.09.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Salmonella typhimurium; gen aidB; nukleotidni ekscizijski popravak
(Salmonella typhimurium; aidB gene; nucleotide excision repair)

Sažetak
The adaptive response of Escherichia coli is an inducible DNA repair system that protects bacterial cells against the mutagenic and cytotoxic effects caused by alkylating agents, particularly by methylating agents. The adaptive response results in the increased expression of four genes, ada, alkA, alkB and aidB. The aidB gene of E. coli is subject of dual regulation, ada-dependent induction by alkylation and ada-independent induction by anaerobiosis or by low pH of growth medium. In contrast to the response in E. coli, an adaptive response is not detectable in Salmonella typhimurium, in spite of the presence of adaST gene in this bacterium. The aim of this study was to examine the presence of the aidB gene in S. typhimurium as well as conditions of its induction. By applying the operon fusion of E. coli aidB gene, its induction by alkylating agents, by anoxia, or by reduced pH has been determined in S. typhimurium and E. coli cells. The impact of nucleotide excision repair on the induction of aidB gene in these two bacteria has been observed. It has been certified for the first time that the aidB DNA fragment from E. coli hybridises with genomic DNA from S. typhimurium. The results of this study further confirm genomic similarity of these two related bacterial species.

Izvorni jezik
Engleski

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