Pregled bibliografske jedinice broj: 26561
Interspecific and interindividual variability of NORs and ITS restriction pattern in Quercus species
Interspecific and interindividual variability of NORs and ITS restriction pattern in Quercus species // Diversity and Adaptation in Oak Species. / Steiner, Kim C. (ur.).
State College (PA): PennState, College of Agricultural Science, 1998. str. 90-97 (predavanje, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)
CROSBI ID: 26561 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Interspecific and interindividual variability of NORs and ITS restriction pattern in Quercus species
Autori
Besendorfer, Višnja ; Zoldoš, Vlatka ; Šiljak-Yakovlev, Sonja ; Javornik, Branka ; Papeš, Dražena
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Diversity and Adaptation in Oak Species.
/ Steiner, Kim C. - State College (PA) : PennState, College of Agricultural Science, 1998, 90-97
Skup
Second meeting of Working Party 2.08.05, Genetics of Quercus, IUFRO
Mjesto i datum
State College (PA), Sjedinjene Američke Države, 12.10.1997. - 17.10.1997
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Quercus; fluorochrome banding; FISH; internal transcribed spacer (ITS); nucleolar organizer region
Sažetak
Fluorochrome banding was employed to characterize oak chromosomes. The karyotypes of three oak species, Quercus robur, Q. petraea and Q. cerris, showed interspecific uniformity of DAPI banding pattern. Chromomycin A3 (CMA) staining revealed CMA-positive bands on several chromosomes and two CMA-positive signals on the satellite chromosome pair. FISH confirmed that they corresponded to nucleolar organizing regions and satellites. Interindividual and even intercellular size variability of CMA-positive signals was observed. Satellites were often detached from the corresponding chromosome pair, and fusion occured occasionally between satellites, either detached or located on the chromosomes. The internal transcribed spacer (ITS) region was successfully amplified in Q. robur, Q. petraea, Q. cerris and Q. pubescens. The PCR-amplified ITS region was approximately 720 bp. Digestion of ITS with Alu I allowed us to distinguish Q. petraea from the other species investigated, while Hae III restriction patterns distinguished Q. robur and Q. cerris from other two species.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
