Naslov
Effect of inactivated Newcastle disease virus given by nebulization to several haematological parameters and immune response of newly-hatched chickens

Autori
Ciglar Grozdanić, Irena ; Šver, Lidija ; Gottstein, Željko ; Židovec-Lepej, Snježana ; Mazija, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
World's Poultry Science Journal, 62 (Suppl.) / Romboli, I. ; Flock, D. ; Franchini, A. - Beekbergen : Personal Media Solutions B. V., 2006

Skup
XII European Poultry Conference

Mjesto i datum
Verona, Italija, 10.09.2006. - 14.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chicken; flow cytometry; inactivated Newcastle Disease Virus; nebulization

Sažetak
The aim of the present study was to investigate the possible induction of immunity in newly-hatched chickens following immunizations with inactivated Newcastle disease virus (NDV) La Sota strain by mean of nebulization using SONOVAC&reg ; 095 device. Additionally, we have studied synergistic and immunomodulating activities of inactivated Parapoxvirus ovis as an immune response modifier (IRM), applied with inactivated antigen. The methods proved to be safe and immunity developed was determinate by HI test in weekly intervals starting from the day of vaccination up to the end of the trial at age of 42 days, and by survival following challenge on 7th, 14th and 21st day post immunization. Although inactivated antigen applied alone or in mixture with IRM, did not induce specific antibodies response, which could detect by HI test, chickens were protected completely following challenges (80-100%). Blood samples for hematological examination and immunophenotyping were taken between day 1 and 18 of age. Statistically significant increase was shown in number of lymphocytes and significant decrease was shown in number of heterophils on 7th day in the immunized chickens. Other hematological parameters as a hematocrit, number of erythrocytes, eosinophils, monocytes, and basophiles did not significant change compare with untreated control group. In order to estimate the immune response to inactivated NDV, the changes of B cell and monocyte compositions in peripheral blood were analyzed by means of flow cytometry using monoclonal antibodies specific for chicken Ia, Bu-1 and IgA cell surface molecules. Specific immunization induced remarkable increase of B-cells and monocyte on day 3. Further investigations of immune response to inactivated NDV given by nebulization deep into respiratory system should be include the use of more effective immunomodulator and the research of antigen-specific immune response in the lungs to understand better host immunological changes associated with immunization by inactivated antigen.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA