Naslov
The influence of growth conditions on induction of the aidB gene in Escherichia coli and Salmonella typhimurium

Autori
Perić, Nataša ; Ivanišević, Miroslava ; Bačun-Družina, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka / Marić, Vladimir - Zagreb : Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 1998

Skup
3. Hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista

Mjesto i datum
Zagreb, Hrvatska, 10-12.06

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Escherichia coli; Salmonella typhimurium; adaptivni odgovor; gen aidB
(Escherichia coli; Salmonella typhimurium; adaptive response; aidB gene)

Sažetak
In order to ensure the survival in their natural environments, bacteria must aadpt to the changing of growth conditions (e.g. variability in temperature and pH, nutrient and oxygen limitation, oxidative agents). Bacterial cells have developed a network of adaptive responses as o overcome these adverse conditions. In Escherichia coli, aidB gene expression is induced in response to three different environmental stimuli: alkylation damage of DNA, anaerobiosis and low pH. In contrast to the response in E. coli, an adaptive response is not detectable in Salmonella typhimurium. Our attention was focused on the induction of the aidB gene in E. coli and S. typhimurium by alkylating agents, anoxia, and reduced pH. The induction of aidB gene was monitored by assaying beta-galactosidase activity in extracts obtained from strains containing the operon fusion of E. coli aidB gene. The results showed that the aidB gene induction responded to unaerated growth condition as well as to acidification of growth media in both E. coli and S. typhimurium.

Izvorni jezik
Engleski

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