Naslov
Expression of visinin-like protein-3 in mouse kidney

Autori
Pribanić, S. ; Loffing, J. ; Madjdpour, C. ; Bačić, Deša ; Gisler, S. ; Braunewell, K.H. ; Biber, J. ; Murer, H.

Izvornik
Nephron Physiology (1660-2137) 95 (2003); 76-82

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Interacting proteins; Na/Pi cotransport; yeast two-hybrid; neuronal calcium sensors

Sažetak
In renal proximal brush borders the Na/Pi cotransporter NaPi-IIa is part of a heteromultimeric complex including the PDZ proteins PDZK1 and NHERF1, which interact with the C terminus of NaPi- IIa. In this study, a yeast two-hybrid screen against the N terminus of the Na/Pi cotransporter NaPi-IIa was performed. Thereby we identified visinin-like protein-3 (VILIP-3), a member of neuronal calcium sensors. In this study, expression and protein localization of VILIP-3 in the mouse kidney was performed by immunofluorescence and RT-PCR using laser- assisted microdissected nephron segments. VILIP-3 was found to be abundant in distal and collecting ducts where it partly colocalized with calbindin D28K. In addition VILIP-3 was observed in the brush borders of proximal tubular S1 and S3 segments of both superficial and deep nephrons.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA