Pregled bibliografske jedinice broj: 26464
RAPD analyses, genome size and genomic in situ hybridization in triploid viviparous onions Ljutika and Pran
RAPD analyses, genome size and genomic in situ hybridization in triploid viviparous onions Ljutika and Pran // Abstracts of the 13th International Chromosome Conference ; u: Cytogenetics and Cell Genetics 81 (1998) (Special Issue 2) 91-168 / Olmo, Ettore (ur.).
Basel: Karger Publishers, 1998. str. 145-145 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 26464 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
RAPD analyses, genome size and genomic in situ hybridization in triploid viviparous onions Ljutika and Pran
Autori
Puizina, Jasna ; Javornik, Branka ; Bohanec, Borut ; Klein, Franz ; Schweizer, Dieter ; Maluszynska, Jolanta ; Papes, Dražena
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Abstracts of the 13th International Chromosome Conference ; u: Cytogenetics and Cell Genetics 81 (1998) (Special Issue 2) 91-168
/ Olmo, Ettore - Basel : Karger Publishers, 1998, 145-145
ISBN
978-3-8055-6759-6
Skup
International Chromosome Conference (13 ; 1998)
Mjesto i datum
Ancona, Italija, 08.09.1998. - 12.09.1998
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
onions Ljutika and Pran; RAPD analyses; genome size; in situ hybridization; triploid viviparous
Sažetak
Identical Randomyl Amplified Polymorphic DNA (RAPD) banding pattern and the same DNA amount (2C = 43, 4 pg) confirmed the high genetic similarity and the unique origin of the two triploid viviparous onions (Alllium cepa L. var. viviparum Metzg. (Alef.), auct.) the Croatian Ljutika and the Indian Pran. In order to elucidate the parental Allium species of these natural triploid hybrids, genomic fluorescent in situ hybridization was applied. Biotinylated genomic DNAs from six diploid Allium species (A. cepa L., A. fistulosum L., A. roylei Stearn, A. vavilovii M. Pop. et Vved., A. galanthum Kar. et Kir., A. oschaninii O. Fedtsch.) were used as probes in this study. The DNA probe from A. cepa labelled completely only one (C) genome (8 longer chromosomes). The DNA probe from A. roylei labelled completely another, (R) genome (8 rather short chromosomes), and one or both telomeric regions of at least 10 other chromosomes. In a few chromosomes the markers of the triploid karyotype were not completely labelled neither with A. cepa DNA probe, nor with A. roylei DNA probe. The results unambigously showed identity of two parental genomes (C and R), whereas the third genome (X) still needs to be identified.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
