ࡱ> ceb7 l{bjbjUU .7|7|`&lLLLLLLL`8<2D`E("" hjjjjjj$ XqLXLLXXXLLhXhXXDLLDv 0;\`^(DD$0EDsrsDX``LLLLGrowth of juvenile striped seabream, Lithognathus mormyrus (Teleostei: Sparidae) in the Adriatic Sea Sanja Mati-Skoko, Josipa Ferri, Miro Kraljevi and Jakov Dul i Institute of Oceanography and Fisheries; Meatrovievo `et. 63, P.O.Box. 500, 21000 Split, Croatia Fax: +385 21 358 650 Corresponding author:  HYPERLINK "mailto:sanja@izor.hr" sanja@izor.hr Summary Growth of juvenile Lithognathus mormyrus from the Due Glava, eastern Adriatic Sea, was analysed. A total of 2133 juveniles, ranging in total lenght from 0.8 to 10.3 cm, were caught. Most individuals (99.53%) belonged to the 0+ cohort. The first settlers, aged 1.5-2.0 months, were recorded at the end of August. Relationship between total length and weight indicates positive allometric growth (b = 3.141). The condition factor, as a consequence of length-weight relationship, was CF = 1.245. The Gompertz (c = 0.080 mm day-1; R2 = 0.962) and linear equation (c = 0.051 mm day-1; R2 = 0.956) seem to be the most appropriate for the description of young L. mormyrus growth. Introduction The larval period is profoundly important in the life of fishes. During this time the new cohort is reduced dramatically to the rare survivor that goes on to reproduce and add to the populations persistence (Jones, 2002). Growth which estimation can provide a descriptive parameter of the conditions surrounding a population (Planes et al., 1999), is the most sensitive parameter to enviromental conditions (Weatherly and Gill, 1987). Using the analysis of size-frequency distributions (Barry and Tegner, 1989), growth of juveniles can be estimated from the increase of the mean size of fish in a cohort within year-classes (Planes et al., 1999). Different growth models can be used to construct growth curve. Data on juvenile fish growth rates are still scarce in the literature, although they are crucial for a better understanding of species population dynamics (Lasker, 1985). The striped seabream Lithognathus mormyrus is a demersal marine fish belonging to the Sparidae family. It is widely distributed in the Mediterranean (absent in the Black Sea), Atlantic (from Bay of Biscay to Cape of Good Hope), Red Sea and southwestern Indian ocean (Bauchot and Hureau, 1986), occurring predominatly over unvegetated sand habitats (Guidetti, 2000). L. mormyrus is a gregarious species, entering exceptionally in coastal lagoons (Suau, 1970; Tortonese, 1975). In spite wide distribution, juvenile striped seabream has never been the object of intensive investigation. Majority of published studies deal with aspects of adult L. mormyrus reproduction, age and growth so these have been studied in the centraleast Atlantic (Lorenzo et al., 2002; Pajuelo et al., 2002), in eastern Spanish coastal waters (Suau, 1955, 1970), in Thracian Sea, Greece (Kallianiotis et al., 2005) and in the northern and middle Adriatic Sea (Kraljevi et al., 1995; Kraljevi et al., 1996). Froglia (1977), Jardas (1985) and Badalamenti et al. (1992) studied feeding habits of adult striped seabream. It is also known that fecundity of L. mormyrus greatly increased from May to June, reaching a maximum at the beginning of June (Suau, 1970) and that this species is a proteandric hermaphrodite (Besseau, 1990). However, nothing is known about the growth of juvenile striped sea bream in the Adriatic Sea and other areas of its wide distribution. Therefore, the aim of the present work was to estimate growth of juvenile L. mormyrus from the Croatian waters, and to test different models for estimating juvenile fish growth. Materials and mathods Due Glava (43262 33 N 16412 E) is a small south-facing sandy beach on the Croatian Adriatic coast (~ 20 km south of Split) within the River Cetina estuary. The sampling area was characteristically sandy and partially overgrown with Cystoseira barbata and Ulva rigida. Samples were collected monthly from April 2000 to March 2001. Fish samples were collected using a 22 m long beach seine with wings of 7.5 m and central collecting area of 7 m. The outer wings were of 4 mm mesh size and the central sac of 2 mm. The net was always hauled from the depth (max. 2 m depth) to the shallow. Collected striped seabream juveniles were preserved in 4% buffered formalin, identified according to Jardas (1996). Due to the time of spawning and the period of intensive settlement, together with the duration of the larval stage of life (Raventos and Macpherson, 2001), which varies between one and two months, July 1st was assumed to be the birth date of striped seabream. Age in months was determined as the difference between the date of capture and the birth date. In the present paper, the term cohort was used to describe groups of L. mormyrus of similar size, identified monthly in each lenght-frequency distribution. Empirical total size-frequency distributions were used to construct an age-lenght key with 3 mm lenght intervals. The commonly used lenght-weight relationship (W=aLtb) and condition factor (CF=100WLt-3) were applied (Ricker, 1979). Several growth models were tested: linear (Lt=a+ct), exponential (Lt=aect), logarithmic (Lt=cLn(t)-a), parabolic (Lt=ct2+bt+a), von Bertalanffy equation (VBGC) (Lt=L"[1 e-c(t-t EMBED Equation.3 )], Beverton and Holt, 1957) and the generalised Gompertz growth equation (Lt=L"e be[exp-c t)]), Ricker 1979). Abbreviations in upper equations are: Lt = total length (cm) at age t; L0 = total length when t = t0; L" = asymptotic length (cm) at the end of the first growth season (Katsuragava and Ekau, 2003); c = instantaneous growth rate when t = t0; t = age (months from birthday or settlement); a, b = constants. The equation was fitted (all individuals included) by an iterative method with a non-linear (user specified) subroutine (StatSoft, 1996). Calculation of determination coefficient (R2) provided a measure of goodness-of-fit (Sokal and Rohlf, 1981). Results A total of 2133 juveniles of L. mormyrus, ranging from 0.8 to 10.3 cm Lt, were analysed (Table 1). Most individuals (99.53%) belonged to the 0+ cohort. First juveniles of L. mormyrus were sampled (962 individuals, 45.10% of the total sample) at the end of August (August 28th), and ranged in length from 0.8 to 1.6 cm. The total weight of these individuals ranged from 0.01 to 0.13 g. According to the spawning period of striped sea bream, their estimated birth date (July 1st) and the duration of the larval stage, these individuals were probably 1.5 2.0 months old. Juveniles of L. mormyrus were caught throughout the year and they left this area mostly in June. Older individuals (>1+) were recorded in February, March, April and June. The slope (b = 3.141, R2 = 0.996) of the total length-weight regression indicated positive allometric growth. By analysing values of b through length frequencies, 3 periods of negative allometric growth have been determined (Table 2). The condition factor, as a consequence of the length-weight relationship, was CF = 1.245. Linear regression of the mass and length logarithm values for every length group hasnt provided the uninterrupted order of dots lying on one straight line. It has, actually, provided few fractures, or straight lines corresponding to the physiological changes during the early stages of the striped sea bream (Fig. 1). The results of using several different growth models are presented in Table 3. All tested models gave different results. Among them, age length data of the juvenile striped sea bream were well described by the growth curve of the Gompertz (c = 0.080 mm day1; R2 = 0.962; Fig. 2) and linear equation (c = 0.051 mm day-1; R2 = 0.956; Fig. 3). Results of the growth increment analysis showed that growth rate of juvenile L. mormyrus increased during the first five months of life although in the period after settlement, in September, slower growth rates were noted. After first five months, in January (mean 12.33C) and in February (mean 12.31C), the limited growth was noted. In the period after February, new growth increment was recorded. The growth slope was highest in October (Fig. 4a). Therefore, few inflection points were evident, indicating the sigmoid growth of juvenile L. mormyrus. The Gompertz and von Bertalanffy models, as usually used, fit the data very well in first five months of life. After that period, Gompertz model overestimated growth in comparison with the real growth slope less then the von Bertalanffy model underestimated growth (Fig. 4b). The real growth slope was calculated according to monthly mean length values of the cohort. Discussion Growth models are necessary for describing the development of one or a number of populations and interactions with the environment. The choice of an appropriate growth model depends on which species is being studied and also on the aims of the study or the research possibilities (Gamito, 1998). There are numerous contradictory studies dealing with the use of appropriate models for estimating juvenile growth. Analysing the results obtained by applying several growth models to juvenile striped seabream, during the period after settlement, we found that the Gompertz (c = 0.080 mm day-1; R2 = 0.962) and linear equation (c = 0.051 mm day-1; R2 = 0.956) best described juvenile growth of this species according to R and its biology. We have already proposed Gompertz equation for this purpose (Mati, 2001, Mati-Skoko et al., 2004, Mati-Skoko et al., 2006) and it has been previously recommended by several authors (Regner, 1980, Monteiro, 1989, Andrade, 1992; Gamito, 1998). On the other hand, Planes et al. (1999) have recommended linear model for juveniles from Diplodus genus, and while this model gave us relatively good estimation of growth for young L. mormyrus, for other tested sparids obtained results in terms of the determination coefficient were not acceptable. The von Bertalanffy growth equation is widely used for estimating growth of adult individuals. However, the VBGC give poor adjustment in the case of this species by underestimating the value of growth slope during whole investigation period. The VBGC has the disadvantage of not being accurate for describing first growth years with exception for species, which estimated t0 doesnt differ from zero, such as Diplodus annularis (Gordoa and Mol, 1997). For species with t0 different to zero, Gordoa and Mol (1997) recommended use of the exponential model. But, obtained curves by both, exponential and logarithmic models didnt follow real growth of analysed species. As a conclusion, a combination of Gompertz equation to describe the first year of growth, with the seasonal von Bertalanffy model to describe the following years, might be the outlet for an adequate description (Gamito, 1998). There is a lack of information about growth of juvenile fish species based on Gompertz models in the literature. Using the Gompertz model, Pallaoro et al. (1998), estimated the growth rate of Oblada melanura to be 0.083 mm day-1 and Gamito (1998) found that Sparus aurata has a growth rate of 0.003 mm day-1. Mati-Skoko et al. (2004) reported about relatively slow growth of juvenile salema, Sarpa salpa (0.047 mm day-1) in the Adriatic, and it is due to the fact that salema settled from ichthyoplankton in November and spent the whole winter in shallow coves with lower temperatures than the open sea. Slower growth of Diplodus puntazzo (0.038 mm day-1) than that of Sarpa salpa can be attributed to the lack of smaller and larger specimens in the sample (Mati-Skoko et al., 2006). Planes et al. (1999) estimated growth rate of sparid juveniles of the genus Diplodus using a linear model and their values are for D. vulgaris c = 0.202 mm day-1 and D. puntazzo c = 0.160 mm day-1. However, it is not recommendable to compare directly growth slopes of different species. The differences in size at age and evident growth rate may be result of methodology imperfections caused by use of small beach seine, slower growth in relatively colder Adriatic Sea (Mati-Skoko et al., 2006) or wrong age interpretation. For that reason, the otolith techniques, which can give us more precise information about the duration of juvenile stage, are welcomed and proposed by Villanueva and Mol (1997). On this sampling area, surface water temperatures follow seasonal cycle with a maximum in July (23.0C) and a minimum in February (12.3C). D. puntazzo, D. vulgaris and S. salpa, which spawn in autumn (Mati, 2001), exhibited lower growth than, related species, such as Diplodus sargus settling in May (Mati-Skoko et al., 2004) and striped seabream settling in August. So, growth is directly and indirectly related with temperature fluctuations. Analysing the values of b through length frequencies, 3 periods of negative allometric growth have been estimated, and they can be connected with periods in the first year in life of juvenile striped sea bream. First period of negative allometric growth was determined in September (Lt=0.6-2.5 cm) in the phase of initial adaptation. Next period (Lt=5.6-7.5 cm) was related to wintertime and low mean temperatures (12.3C). Finally, third period of negative allometric growth (Lt=9.6-10.5 cm) fitted the process of recruitment related with movement from this shallow area and association with adults in deeper waters. Disharmony phases represent crucial periods in the growth of the species. By linear regression, three disharmony phases have been estimated in early life of juvenile striped seabream. Those three phases are settlement, initial adaptation and recruitment, and they are connected with three periods of negative allometric growth and growth increment analyses, also previously mentioned. Settlement intensity of L. mormyrus juveniles in area of Cetina Estuary was highest at the end of August suggesting there was no bigger differences in time when majority of settlers entered in shallow. Knowing the spawning period of this species on the eastern coast of the Adriatic Sea (Kraljevi et al., 1995), it comes out that settlement occurred on whole Adriatic area in almost the same time. Consequently, this small difference in settlement shouldnt have influence growth. Furthermore, only ten adult specimens were found in the investigated area, pointing out that juvenile L. mormyrus do not compete with adults for habitat. These juveniles recruited in very shallow water forming a small number of monospecific shoals. Macpherson (1998) reported that monospecific shoals of such juvenile sparids never mix with the shoals of adults if they were present in the nursery area. Number of L. mormyrus specimens declined from one month to the next one, especially after December when sea temperatures drastically fall down. We presume that most of these individuals have been recruited at about 6-7 cm of total length, changing cold shallow waters for deeper habitats with more stabile conditions. Also, smaller individuals cannot be retained in codend while older, more agile individuals are more capable to avoid net. Therefore, the impacts of sampling by small beach seine on the success of the recruitment have to be clear away. Consequently, using a technique less destroying such as visual census will be more acceptable. Only difficulty may show in size estimation of smaller individuals in first few months after settlement (2-3 cm of total length). In addition, such high decrease of sampled individuals after December may result from natural mortality due to predation (Bailey and Houde, 1989) or starvation. Juveniles that stayed in this shallow area until next summer probably were too small or exhausted to left shallow waters with others in December. There are at least two reasons for the fast juvenile fish growth as this of L. mormyrus: predator avoidance and earlier sexual maturity achievement. For this species hypothesis the bigger is better given by Bailey and Houde (1989) seems to be true. It is evident that earlier recruitment, after age of five or six months, is achieved as a predator avoidance strategy or looking for calmer habitat instead of daily and seasonally varying estuarine areas because L. mormyrus spawned after second year of life (Kraljevi et al., 1995). Shallow waters of Due area are inhabited by species of total length around 20 40 cm (Sparids, Labrids, Mugilids) and since maximal vulnerability was attained when the relative size of the fish larvae was 10% of the predator size (Paradis et al. 1996), it is crucially important for L. mormyrus juveniles to reach lengths of over 40 mm as soon as possible. Future studies should try to determine growth patterns modifications of species at different stages of development caused by different habitat conditions, as was previously proposed by Gordoa and Mol (1997). It is also very important to incorporate juvenile growth into studies of adult population growth. Acknowledgements The authors gratefully acknowledge to Ministry of Science and Technology of Republic Croatia for the financial support of this study through the project Adriatic. Also, we want to acknowledge dr. Melita Peharda (IOR, Croatia) for her efforts in English editing. 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Raventos, N.; Macpherson, E., 2001: Planktonic larval duration and settlement and settlement marks on the otoliths of Mediterranean littoral fishes. Mar. Biol. 138, 1115-1120. Regner, S., 1980: On semigraphic estimation of parameters of Gompertz function and its application on fish growth. Acta Adriat. 21, 227-236. Ricker, W.E., 1979: Growth rates and models. In: Hoar, W.S., Randall, D.J., Brett, J.R. (Eds.), Fish Physiology Vol. VIII, Bioenergetics and Growth. Academic Press, New York, pp. 678-743. Sokal, R.R.; Rohlf, F.J., 1981: Biometry. The principles and practice of statistics in biology research. Freeman and Company, San Francisco, 563 pp. StatSoft, 1996: Statistica for Windows, Tulsa. Suau, P., 1955: Contribucin al studio de la herrera (Pagellus mormyrus L.) especialmente de la sexualidad. Inv. Pesq. 1, 59-66. Suau, P., 1970: Contribucin al studio de la biologia de Lithognathus (=Pagellus) mormyrus L. (Peces espridos). Inv. Pesq. 34, 237-265. Tortonese, E., 1975: Fauna dItalia, XI. Osteichthyes-Pesci ossei. Edizioni Calderini, Bologne, 565 pp. Villanueva, R.; Mol, B., 1997: Validation of the otolith increment deposition ratio using alizarin marks in juveniles of the sparid fishes, Diplodus vulgaris and D. puntazzo. Fish. Res. 30, 257-260. Weatherly, A.H.; Gill, H.S., 1987: The biology of fish growth. Academic Press, London. TABLES 1. The length frequency distribution (with 3 mm intervals) of juvenile L. mormyrus in Croatian waters 2. The results of analysing allometric growth through length frequencies for juvenile L. mormyrus 3. The results of using several different growth models for juvenile L. mormyrus FIGURES 1. Disharmony phases during the early stages of the striped sea bream 2. Gompertz growth curve of juvenile L. mormyrus from the Adriatic Sea 3. Linear growth curve of juvenile L. mormyrus from the Adriatic Sea 4. A. The monthly growth increment analysis of juvenile L. mormyrus; B. 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